TGuide FFPE DNA Imwe-Nhanho Kit

Imwe-nhanho kubviswa kwe genome DNA kubva kuFFPE sampuli.

TGuide FFPE DNA Imwe-Nhanho Kit yakanyatsogadzirirwa kuburitsa genome DNA kubva parafini tishu masampuli neTGuide automated nucleic acid extractors. Iyi kit inoshandisa imwe-nhanho yekupisa nzira, kuitira kuti deparaffinization uye tishu lysis inogona kuitwa panguva imwe chete kuitwe, uye zvinokuvadza reagents senge xylene haina zvirimo. Iyi kit ine maitiro maviri ekubvisa: Kune mashoma masampuli, sarudza maawa maviri lysis; Kune akawanda masampuli, sarudza maawa gumi nematanhatu pahusiku hwe lysis. Iyo kititi inotora cellulose yakadzika magineti emabheji tekinoroji kuti inyatsobvisa genome DNA.

Cat. Aihwa	Kurongedza Saizi
OSR-M405	48 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvishandiso

Yakanatswa DNA inogona kushandiswa zvakananga mukuita kwakasiyana-siyana kwakajairika, kusanganisira enzymatic digestion, PCR, kuvaka raibhurari, Southern blot uye kumwe kuyedza.

Zvimiro

■ In-muchina deparaffinization: Iyo deparaffinization uye yekuchera matanho anogona kupedzwa otomatiki nekungoisa isina kutorwa parafini yakadzamidzirwa sampuli mune reagent katuriji uyezve nekuwedzera Sula Oiri uye Proteinase K.
Results Mhedzisiro inovimbika: Iyo yakawanikwa genome haina mahara kubva kuRNA uye kusvibiswa kweprotein, uye inogona kushandiswa zvakananga kuPCR kana fluorescence huwandu hwePCR.
■ Yakachengeteka uye isingakuvadzi: Organic solvents ayo anokuvadza muviri wemunhu senge phenol chloroform haadiwe.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: TGuide Masero / Tissue Genomic DNA Kit

Zvadaro: TGuide Bhakitiriya Genomic DNA Kit

Mhedzisiro yacho yakaratidza kuti kune makuru evhoriyamu sampuli, iyo yekuburitsa goho dzenzira mbiri dzaifananidzwa; yemasampuli ane epakati uye madiki vhoriyamu, goho reimwe-nhanho deparaffinization nzira (ita neTGuide) yaive yakakura kupfuura iyo yakajairwa deparaffinization nzira. Naizvozvo, iyo-nhanho deparaffinization nzira kwete chete inorerutsa mashandiro, inoderedza njodzi, asi zvakare inovandudza kuburitsa kugona.
Ongorora:
Yakakura vhoriyamu sampuro: iyo nzvimbo yekumisikidza iri mukati me2,5-4 cm² uye ukobvu huri mukati me5-20 μm.
Pakati pevhoriyamu sampuro: iyo nzvimbo yekumisikidza iri mukati me 1.5-2.5 cm² uye ukobvu huri mukati me5-20 μm.
Mudiki vhoriyamu sampuro: iyo nzvimbo yekumisikidza iri mukati me 0.2-1.5 cm² uye ukobvu huri mukati me5-20 μm.

Mubvunzo: Diki kana isina DNA mune iyo eluent.

A-1 Yakadzika kuwanda kwemasero kana hutachiona mune yekutanga sampuro -Pfumisa kuwanda kwemasero kana mavhairasi.

A-2 Kukwana kukosheswa kwemasampuli -Iyo mienzaniso haina kusanganiswa zvakaringana neye lysis buffer. Izvo zvinokurudzirwa kunyatso sanganisa ne pulse-vortexing kwe 1-2 nguva. —Iyo isina kukwana cell lysis inokonzerwa neicho chiitiko kuderera kweproteinase K. - Isina kukwana cell lysis kana protein degradation nekuda kwekushomeka kwekushambidza kwenguva yekugeza. Zvinokurudzirwa kutema zvidimbu zvidimbu zvidiki uye nekuwedzera nguva yekugeza kubvisa ese masara mu lysate.

A-3 Yakakwana DNA adsorption. -Hapana ethanol kana yakaderera-muzana panzvimbo pe100% ethanol yakawedzerwa isati yadzoserwa kune lysate kune spin column.

A-4 Iyo pH kukosha kwe elution buffer yakanyanya kuderera. - Chinja pH kuti ive pakati pe8.0-8.3.

Mubvunzo: DNA haiite mushe murwizi enzymatic reaction experiments.

Residual ethanol mune iyo eluent.

—Pane zvakasara zvekuwacha buffer PW mune yakasviba. Iyo ethanol inogona kubviswa ne centrifuging iyo spin ikholamu ye 3-5 min, uye nekuisa pakamuri tembiricha kana 50 ℃ incubator ye 1-2 min.

Mubvunzo: DNA kudzikisira

A-1 Muenzaniso hausi mutsva. -Tora muenzaniso wakanaka weDNA senzira yekuona kuti DNA iri musampu yanyadzisa here.

A-2 Isina kunaka pre-kurapwa. -Yakakonzerwa nekuwandisa kwemvura senitrogen kukuya, hunyoro hunowanikwa, kana huwandu hwakanyanya hwemuenzaniso.

Mubvunzo: Maitiro ekuitisa prereatment yeiyo gDNA kuburitsa?

Iwo ekufanofanirwa anofanira kusiyana pamasampuli akasiyana. Pamasampuli emiti, ita shuwa yekukuya zvakakwana muinitrogen yemvura. Kune sampuli yemhuka, homogenate kana kunyatso kukuya muinitrogen yemvura. Kune masampuli ane maseru maseru ayo akaoma kutyora, akadai seG + mabhakitiriya uye mbiriso, zvinokurudzirwa kushandisa lysozyme, lyticase kana mechina nzira kuputsa masero masvingo.

Mubvunzo: Ndeupi musiyano uripo pakati pemiti mitatu yekudyara gDNA kits 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Dyara Genomic DNA Kit inotora nzira-yakavakirwa nzira inoda chloroform yekubvisa. Inonyanya kukodzera kune akasiyana masampuli emiti, pamwe neyakaomeswa poda yakaoma. Hi-DNAsecure Plant Kit inoiswawo mumutsetse, asi pasina chikonzero che phenol / chloroform kuburitsa, ichiita kuti ive yakachengeteka uye isiri chepfu. Iyo inokodzera zvirimwa zvine yakakwirira polysaccharides uye polyphenol zvemukati. 4992709/4992710 DNAquick Plant System inotora nzira yemvura-yakavakirwa. Phenol / chloroform kuburitsa hazvidiwe futi. Maitiro ekuchenesa ari nyore uye anokurumidza pasina muganho wemuenzaniso wekutanga, saka vashandisi vanogona kugadzirisa huwandu hwacho nenzira inoenderana nezvinodiwa pakuedza. Kukura kukuru kwezvimedu zveGDNA zvinogona kuwanikwa nezvibereko zvakakura.

Ndezvipi zvibereko zvinofungidzirwa zveGDNA kubva 1 ml ropa sampuro neTIANamp Ropa reDNA Kit?

Iyo genomic DNA yakaburitswa kubva kumavhoriyamu akasiyana eropa rese revanhu neTIANamp Ropa reDNA Kit. Mhedzisiro yacho ndeinotevera. Mhedzisiro yacho yakanyorwa sereferenzi chete, iyo chaiyo mhedzisiro mhedzisiro inoenderana nemamiriro esampuli.

Mubvunzo: 4992207/4992208 uye 4992722/4992723 ingashandiswe kubvisa ropa rakagwamba DNA?

Ropa rinovhara ropa reDNA rinogona kuitwa pachishandiswa reagents yakapihwa mumakiti maviri aya nekungochinja protocol kune yakatarwa murau reropa rekuvhara ropa reDNA. Iyo yakapfava kopi yeropa clot DNA yekubvisa protocol inogona kupihwa pakukumbira.

Mubvunzo: Kana uchizora TIANamp Genomic DNA Kit, maitiro ekutyora matishu matsva museru kuturika?

Misa svumbunuro nyowani ne 1 ml PBS, yakasarudzika saline kana TE buffer. Zvakanyatso homogenize muyenzaniso ne homogenizer uye unganidza iyo inodzika pasi pezere pombi ne centrifuging. Dzinga iyo inoshamisa, uye dzorerazve iyo precipitate ne200 μl buffer GA. Iyo inotevera yekunatswa kweDNA inogona kuitwa maererano nemirairo.

Mubvunzo: Ungasarudza sei chigadzirwa cheiyo DNA kubviswa kubva muplasma, serum uye nemuviri maseru sampu?

Zvekucheneswa kweGDNA muplasma, serum uye muviri fluid sampuro, TIANamp Micro DNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kuSerum / plasma sampuro, TIANamp Virus DNA / RNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kune serum uye plasma sampuro, TIANamp Bacteria DNA Kit inokurudzirwa (lysozyme inofanirwa kuverengerwa kune yakanaka bhakitiriya). Zvematehwe sampu, Hi-Swab DNA Kit uye TIANamp Bacteria DNA Kit zvinokurudzirwa.

Mubvunzo: Ungasarudza sei makiti eiyo gDNA kubviswa kubva kune fungi sampuli?

DNAsecure Plant Kit kana DNAquick Plant System inokurudzirwa kubviswa kwefungal genome. Nezve mbiriso genome kuburitsa, TIANamp Yeast DNA Kit inokurudzirwa (lyticase inofanira kuzvigadzirira).

Cell Yemahara Dna Kubvisa Kit