TGuide Virus DNA / RNA Kit

Yekutora hutachiona DNA / RNA kubva kune serum, plasma, isina-sero muviri fluid kana gadziriso yekuchengetedza hutachiona.

TGuide Virus DNA / RNA Kit yakanyatsogadzirirwa kuburitsa nucleic acid kubva kuhutachiona uchishandisa TGuide Series Automated Nucleic Acid Extractor. Zvishandiso zvepurasitiki zvinoshandiswa mukiti zvakarapwa kubvisa DNase / RNase, uye yega sampuro inomhanya yakazvimiririra. Iyo sisitimu inogona kunyatso kudzivirira mikana dzakasiyana dzekuyambuka-kusvibiswa pakati pemasampuli. Iyo kit inogona kutora hutachiona DNA kana RNA kubva ku200 μl / 400 μl masampuli panguva imwe chete. Zviri mari uye nyore kushandisa.

Cat. Aihwa	Kurongedza Saizi
OSR-M202	48 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvishandiso

Iyo yakanatswa nucleic acid inokodzera yakakwira senitivity PCR, RTPCR, huwandu hwePCR, huwandu hweRT-PCR uye kumwe kuyedza. Iyo kit yakave yakasimbiswa nekuderera kwekudzika kweHBV, HCV, HIV uye hutachiona hutachiona.

Zvimiro

■ Rakareruka uye rinokurumidza kuburitsa: TGuide zvigadzirwa zvekushandisa zvinovakirwa pamisimboti yeiyo nucleic acid yekuchenesa nemagineti marozari, uye hutachiona hwenhengo yeacicicic yekubvisa maitiro inogona kupedzwa mumaminetsi makumi mashanu nemanomwe.
■ Hapana kusvibiswa: Makiratjhini akazvimiririra akavharirwa reagent akafanorongedzwa kudzivirira hutachiona.
■ Goho repamusoro: Iyo kit ine yepamusoro-soro Yekutakura RNA yekuvandudza mashandiro eiyo nucleic acid kubatwa.
No Hapana phenol uye chloroform inoburitswa: Hapana zvinogadziriswa zvinokanganisa muviri wemunhu zvakaita se phenol uye chloroform zvinodiwa.
■ Flexible sampuli yekutanga huwandu: 200 μl / 400 μl sampuli dzinogona kuburitswa zvakananga nemakiti anoenderana.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: TGuide Plasma DNA Kubvisa Kit (1.2ml)

Zvadaro: TGuide Kudyara Genomic DNA Kit

Fluorescence yakawandisa PCR kuona kweHBV

Mufananidzo 1: Chenesa 200 μl yakanaka murwere serum ine HBV yenzvimbo dzakasiyana dzichishandisa TGuide Virus DNA / RNA Kit. Iyo yakawanikwa HBV hutachiona hweDNA yakatsakatika mu60 μl elution buffer.
Yakavezwa PCR kuona kweHCV

Mufananidzo 2: Bvisa masamu esamu ane huwandu hwakasiyana hwehutachiona hweHCV neTGuide Virus DNA / RNA Kit, uye uone mhedzisiro nePCR yakavezwa.
1: 5 × 10⁰ HCV Serum 2: 5 × 10¹ HCV Serum
3: 5 × 10² HCV Serum 4: 5 × 10³ HCV Serum
5: 5 × 10⁴ HCV Serum 6: 5 × 10⁵ HCV Serum
P: Positive control N: Negative kudzora
M: 100 bp DNA manera

Mubvunzo: Diki kana isina DNA mune iyo eluent.

A-1 Yakadzika kuwanda kwemasero kana hutachiona mune yekutanga sampuro -Pfumisa kuwanda kwemasero kana mavhairasi.

A-2 Kukwana kukosheswa kwemasampuli -Iyo mienzaniso haina kusanganiswa zvakaringana neye lysis buffer. Izvo zvinokurudzirwa kunyatso sanganisa ne pulse-vortexing kwe 1-2 nguva. —Iyo isina kukwana cell lysis inokonzerwa neicho chiitiko kuderera kweproteinase K. - Isina kukwana cell lysis kana protein degradation nekuda kwekushomeka kwekushambidza kwenguva yekugeza. Zvinokurudzirwa kutema zvidimbu zvidimbu zvidiki uye nekuwedzera nguva yekugeza kubvisa ese masara mu lysate.

A-3 Yakakwana DNA adsorption. -Hapana ethanol kana yakaderera-muzana panzvimbo pe100% ethanol yakawedzerwa isati yadzoserwa kune lysate kune spin column.

A-4 Iyo pH kukosha kwe elution buffer yakanyanya kuderera. - Chinja pH kuti ive pakati pe8.0-8.3.

Mubvunzo: DNA haiite mushe murwizi enzymatic reaction experiments.

Residual ethanol mune iyo eluent.

—Pane zvakasara zvekuwacha buffer PW mune yakasviba. Iyo ethanol inogona kubviswa ne centrifuging iyo spin ikholamu ye 3-5 min, uye nekuisa pakamuri tembiricha kana 50 ℃ incubator ye 1-2 min.

Mubvunzo: DNA kudzikisira

A-1 Muenzaniso hausi mutsva. -Tora muenzaniso wakanaka weDNA senzira yekuona kuti DNA iri musampu yanyadzisa here.

A-2 Isina kunaka pre-kurapwa. -Yakakonzerwa nekuwandisa kwemvura senitrogen kukuya, hunyoro hunowanikwa, kana huwandu hwakanyanya hwemuenzaniso.

Mubvunzo: Maitiro ekuitisa prereatment yeiyo gDNA kuburitsa?

Iwo ekufanofanirwa anofanira kusiyana pamasampuli akasiyana. Pamasampuli emiti, ita shuwa yekukuya zvakakwana muinitrogen yemvura. Kune sampuli yemhuka, homogenate kana kunyatso kukuya muinitrogen yemvura. Kune masampuli ane maseru maseru ayo akaoma kutyora, akadai seG + mabhakitiriya uye mbiriso, zvinokurudzirwa kushandisa lysozyme, lyticase kana mechina nzira kuputsa masero masvingo.

Mubvunzo: Ndeupi musiyano uripo pakati pemiti mitatu yekudyara gDNA kits 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Dyara Genomic DNA Kit inotora nzira-yakavakirwa nzira inoda chloroform yekubvisa. Inonyanya kukodzera kune akasiyana masampuli emiti, pamwe neyakaomeswa poda yakaoma. Hi-DNAsecure Plant Kit inoiswawo mumutsetse, asi pasina chikonzero che phenol / chloroform kuburitsa, ichiita kuti ive yakachengeteka uye isiri chepfu. Iyo inokodzera zvirimwa zvine yakakwirira polysaccharides uye polyphenol zvemukati. 4992709/4992710 DNAquick Plant System inotora nzira yemvura-yakavakirwa. Phenol / chloroform kuburitsa hazvidiwe futi. Maitiro ekuchenesa ari nyore uye anokurumidza pasina muganho wemuenzaniso wekutanga, saka vashandisi vanogona kugadzirisa huwandu hwacho nenzira inoenderana nezvinodiwa pakuedza. Kukura kukuru kwezvimedu zveGDNA zvinogona kuwanikwa nezvibereko zvakakura.

Ndezvipi zvibereko zvinofungidzirwa zveGDNA kubva 1 ml ropa sampuro neTIANamp Ropa reDNA Kit?

Iyo genomic DNA yakaburitswa kubva kumavhoriyamu akasiyana eropa rese revanhu neTIANamp Ropa reDNA Kit. Mhedzisiro yacho ndeinotevera. Mhedzisiro yacho yakanyorwa sereferenzi chete, iyo chaiyo mhedzisiro mhedzisiro inoenderana nemamiriro esampuli.

Mubvunzo: 4992207/4992208 uye 4992722/4992723 ingashandiswe kubvisa ropa rakagwamba DNA?

Ropa rinovhara ropa reDNA rinogona kuitwa pachishandiswa reagents yakapihwa mumakiti maviri aya nekungochinja protocol kune yakatarwa murau reropa rekuvhara ropa reDNA. Iyo yakapfava kopi yeropa clot DNA yekubvisa protocol inogona kupihwa pakukumbira.

Mubvunzo: Kana uchizora TIANamp Genomic DNA Kit, maitiro ekutyora matishu matsva museru kuturika?

Misa svumbunuro nyowani ne 1 ml PBS, yakasarudzika saline kana TE buffer. Zvakanyatso homogenize muyenzaniso ne homogenizer uye unganidza iyo inodzika pasi pezere pombi ne centrifuging. Dzinga iyo inoshamisa, uye dzorerazve iyo precipitate ne200 μl buffer GA. Iyo inotevera yekunatswa kweDNA inogona kuitwa maererano nemirairo.

Mubvunzo: Ungasarudza sei chigadzirwa cheiyo DNA kubviswa kubva muplasma, serum uye nemuviri maseru sampu?

Zvekucheneswa kweGDNA muplasma, serum uye muviri fluid sampuro, TIANamp Micro DNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kuSerum / plasma sampuro, TIANamp Virus DNA / RNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kune serum uye plasma sampuro, TIANamp Bacteria DNA Kit inokurudzirwa (lysozyme inofanirwa kuverengerwa kune yakanaka bhakitiriya). Zvematehwe sampu, Hi-Swab DNA Kit uye TIANamp Bacteria DNA Kit zvinokurudzirwa.

Mubvunzo: Ungasarudza sei makiti eiyo gDNA kubviswa kubva kune fungi sampuli?

DNAsecure Plant Kit kana DNAquick Plant System inokurudzirwa kubviswa kwefungal genome. Nezve mbiriso genome kuburitsa, TIANamp Yeast DNA Kit inokurudzirwa (lyticase inofanira kuzvigadzirira).

Cell Yemahara Dna Kubvisa Kit