TGuide Ropa Genomic DNA Kit

Yekubvisa genomic DNA kubva kuvanhu kana mammalian ropa rese.

TGuide Ropa Genomic DNA kit yakanyatsogadzirirwa kuburitsa DNA (inosanganisira genomic DNA, mitochondrial DNA uye hutachiona DNA) kubva muropa rose, serum, plasma uye leukocytes vachishandisa TGuide Series Automated Nucleic Acid Extractor. Reagents inodiwa kune cell lysis uye protein degradation, iyo yemagineti marozari anonyatso adsorbing DNA, yekuwacha buffer, nezvimwe zvakatotakurwa-mu reagent makatiriji. Iyo yakanatswa DNA ichave yakasarudzika mune yakaderera-munyu buffer. Kureba kwe genomic DNA yakanatswa nekiti ndeye 20- 30 kb, inoenderana nePCR kana imwe enzymatic reaction.

Cat. Aihwa	Kurongedza Saizi
OSR-M102	48 preps
OSR-M102-T1	48 preps
OSR-M104	48 preps
OSR-M104-T1	48 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Ongororo Muenzaniso 1

Muenzaniso Wokuongorora 2

Muenzaniso Wokuongorora 3

MIBVUNZO

Chigadzirwa Mateki

Zvishandiso

Iyo yakanatswa genome inogona kushandiswa zvakananga muPCR, RT-PCR, enzyme digestion reaction, Maodzanyemba hybridization uye kumwe kuyedza.

Zvimiro

■ Rakareruka uye rinokurumidza kuburitsa: TGuide zvishongedzo zvigadzirwa zvinovakirwa pamisimboti yeiyo nucleic acid yekuchenesa nemagineti marozari, uye genomic DNA kuburitsa kunogona kupedziswa mu44 min.
■ Mhedzisiro yakavimbika: Iyo genomic DNA yakaburitswa nekiti haina tsvina seRNA neprotein, uye inogona kushandiswa zvakananga kuPCR kana fluorescence yakawandisa PCR.
No Hapana phenol uye chloroform inoburitswa: Hapana zvinogadziriswa zvinokanganisa muviri wemunhu zvakaita se phenol uye chloroform zvinodiwa.
F Saizi inoshanduka yekutanga saizi: 200 μl, 400 μl, 1.2 ml yeropa rese kana 1-3 ml yeleukocyte isati yatsemurwa inogona kuburitswa zvakananga nekusarudza kit inowirirana.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: TIANSeq rRNA Kubvisa Kit (H / M / R)

Zvadaro: TGuide Plasma DNA Kubvisa Kit (1.2ml)

	Mhedzisiro ye genomic DNA yakatorwa kubva ku200 μl ropa rese rakarapwa nemaanticoagulants akasiyana
	DNA yakanyungudutswa mu200 μl elution buffer. M: 1 kb manera 1-5 Kuonekwa kweanhibitors akasiyana anticoagulants. Target gene: Cbl-b geni, 1.7 kb P: Kudzora kwakanaka

	Mhedzisiro ye genomic DNA yakatorwa kubva ku400 μl kana 600 μl yakazara ropa
	DNA yakanyungudutswa mu100 μl elution buffer Genomic DNA yakabviswa kubva pa400 μl kana 600 μl muropa rose M: DL15000 Maka Kuisa vhoriyamu: 2 μl

	Mhedzisiro ye genomic DNA yakatorwa kubva ku1.2 ml ropa rese
	DNA yakanyungudutswa mu300 μl elution buffer Genomic DNA yakabviswa kubva pa1.2 ml ropa rese M: DL15000 Maka; Kuisa vhoriyamu: 2 μl

Mubvunzo: Diki kana isina DNA mune iyo eluent.

A-1 Yakadzika kuwanda kwemasero kana hutachiona mune yekutanga sampuro -Pfumisa kuwanda kwemasero kana mavhairasi.

A-2 Kukwana kukosheswa kwemasampuli -Iyo mienzaniso haina kusanganiswa zvakaringana neye lysis buffer. Izvo zvinokurudzirwa kunyatso sanganisa ne pulse-vortexing kwe 1-2 nguva. —Iyo isina kukwana cell lysis inokonzerwa neicho chiitiko kuderera kweproteinase K. - Isina kukwana cell lysis kana protein degradation nekuda kwekushomeka kwekushambidza kwenguva yekugeza. Zvinokurudzirwa kutema zvidimbu zvidimbu zvidiki uye nekuwedzera nguva yekugeza kubvisa ese masara mu lysate.

A-3 Yakakwana DNA adsorption. -Hapana ethanol kana yakaderera-muzana panzvimbo pe100% ethanol yakawedzerwa isati yadzoserwa kune lysate kune spin column.

A-4 Iyo pH kukosha kwe elution buffer yakanyanya kuderera. - Chinja pH kuti ive pakati pe8.0-8.3.

Mubvunzo: DNA haiite mushe murwizi enzymatic reaction experiments.

Residual ethanol mune iyo eluent.

—Pane zvakasara zvekuwacha buffer PW mune yakasviba. Iyo ethanol inogona kubviswa ne centrifuging iyo spin ikholamu ye 3-5 min, uye nekuisa pakamuri tembiricha kana 50 ℃ incubator ye 1-2 min.

Mubvunzo: DNA kudzikisira

A-1 Muenzaniso hausi mutsva. -Tora muenzaniso wakanaka weDNA senzira yekuona kuti DNA iri musampu yanyadzisa here.

A-2 Isina kunaka pre-kurapwa. -Yakakonzerwa nekuwandisa kwemvura senitrogen kukuya, hunyoro hunowanikwa, kana huwandu hwakanyanya hwemuenzaniso.

Mubvunzo: Maitiro ekuitisa prereatment yeiyo gDNA kuburitsa?

Iwo ekufanofanirwa anofanira kusiyana pamasampuli akasiyana. Pamasampuli emiti, ita shuwa yekukuya zvakakwana muinitrogen yemvura. Kune sampuli yemhuka, homogenate kana kunyatso kukuya muinitrogen yemvura. Kune masampuli ane maseru maseru ayo akaoma kutyora, akadai seG + mabhakitiriya uye mbiriso, zvinokurudzirwa kushandisa lysozyme, lyticase kana mechina nzira kuputsa masero masvingo.

Mubvunzo: Ndeupi musiyano uripo pakati pemiti mitatu yekudyara gDNA kits 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Dyara Genomic DNA Kit inotora nzira-yakavakirwa nzira inoda chloroform yekubvisa. Inonyanya kukodzera kune akasiyana masampuli emiti, pamwe neyakaomeswa poda yakaoma. Hi-DNAsecure Plant Kit inoiswawo mumutsetse, asi pasina chikonzero che phenol / chloroform kuburitsa, ichiita kuti ive yakachengeteka uye isiri chepfu. Iyo inokodzera zvirimwa zvine yakakwirira polysaccharides uye polyphenol zvemukati. 4992709/4992710 DNAquick Plant System inotora nzira yemvura-yakavakirwa. Phenol / chloroform kuburitsa hazvidiwe futi. Maitiro ekuchenesa ari nyore uye anokurumidza pasina muganho wemuenzaniso wekutanga, saka vashandisi vanogona kugadzirisa huwandu hwacho nenzira inoenderana nezvinodiwa pakuedza. Kukura kukuru kwezvimedu zveGDNA zvinogona kuwanikwa nezvibereko zvakakura.

Ndezvipi zvibereko zvinofungidzirwa zveGDNA kubva 1 ml ropa sampuro neTIANamp Ropa reDNA Kit?

Iyo genomic DNA yakaburitswa kubva kumavhoriyamu akasiyana eropa rese revanhu neTIANamp Ropa reDNA Kit. Mhedzisiro yacho ndeinotevera. Mhedzisiro yacho yakanyorwa sereferenzi chete, iyo chaiyo mhedzisiro mhedzisiro inoenderana nemamiriro esampuli.

Mubvunzo: 4992207/4992208 uye 4992722/4992723 ingashandiswe kubvisa ropa rakagwamba DNA?

Ropa rinovhara ropa reDNA rinogona kuitwa pachishandiswa reagents yakapihwa mumakiti maviri aya nekungochinja protocol kune yakatarwa murau reropa rekuvhara ropa reDNA. Iyo yakapfava kopi yeropa clot DNA yekubvisa protocol inogona kupihwa pakukumbira.

Mubvunzo: Kana uchizora TIANamp Genomic DNA Kit, maitiro ekutyora matishu matsva museru kuturika?

Misa svumbunuro nyowani ne 1 ml PBS, yakasarudzika saline kana TE buffer. Zvakanyatso homogenize muyenzaniso ne homogenizer uye unganidza iyo inodzika pasi pezere pombi ne centrifuging. Dzinga iyo inoshamisa, uye dzorerazve iyo precipitate ne200 μl buffer GA. Iyo inotevera yekunatswa kweDNA inogona kuitwa maererano nemirairo.

Mubvunzo: Ungasarudza sei chigadzirwa cheiyo DNA kubviswa kubva muplasma, serum uye nemuviri maseru sampu?

Zvekucheneswa kweGDNA muplasma, serum uye muviri fluid sampuro, TIANamp Micro DNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kuSerum / plasma sampuro, TIANamp Virus DNA / RNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kune serum uye plasma sampuro, TIANamp Bacteria DNA Kit inokurudzirwa (lysozyme inofanirwa kuverengerwa kune yakanaka bhakitiriya). Zvematehwe sampu, Hi-Swab DNA Kit uye TIANamp Bacteria DNA Kit zvinokurudzirwa.

Mubvunzo: Ungasarudza sei makiti eiyo gDNA kubviswa kubva kune fungi sampuli?

DNAsecure Plant Kit kana DNAquick Plant System inokurudzirwa kubviswa kwefungal genome. Nezve mbiriso genome kuburitsa, TIANamp Yeast DNA Kit inokurudzirwa (lyticase inofanira kuzvigadzirira).

Cell Yemahara Dna Kubvisa Kit