■ Zviratidzo zvemavara zvinowedzerwa kuma reagents kunzvenga mapoinzi ekuwedzera zvikanganiso kana kusiiwa nekuda kwenhamba huru yemasampuli.
■ Kukosha kweye fluorescence, kunzwisisika kwepamusoro uye kusanyatsojeka.
After Mushure mekuongororwa kwakasimba, chiratidzo hachizokanganisa kukosha kweiyo fluorescence uye nemhedzisiro yeiyo dhayi.
■ Inokodzera akasiyana akasiyana enguva chaiyo PCR zviridzwa (Roche, Bio-rad, ABI, nezvimwewo).
Iyo inokodzera mhando dzakasiyana dzekuyedza senge kutaura kwekuongorora uye nucleic acid kuonekwa neSYBR Green nzira pane dzakasiyana nguva chaidzo PCR zviridzwa. Inonyanya kukodzera kuyedzwa kwepamusoro kwekuyedza nenhamba yakawanda yemasampuli uye yakaoma sampuro yekurodha mashandiro.
Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)
Mufananidzo. 1. Magirafu eamplififi cation curves uye akanyungudika mac curves anowanikwa nekuteedzera huwandu hwevanhu HS1 geni uchishandisa TIANGEN SuperReal PreMix Plus (SYBR Green) (A) uye SuperReal PreMix Ruvara (SYBR Green) (B). Iyo template yakadzoserwa for 5 concentration gradients. Iwo maCt maitiro (C) akawanikwa nekuenzanisa anoratidza kuti kukwidziridzwa kwemakiti maviri ane kunzwisisika kwakanyanya, kujeka uye kugadzikana. Kuedza kwacho kwakaitwa paRoche LightCycler480. | |
Mufananidzo 2. Girafu rekusimudzira curve uye yakanyunguduka curve inowanikwa nekuteedzera huwandu hwevanhu HS5 geni uchishandisa TIANGEN SuperReal PreMix Ruvara (SYBR Green) uye chakakodzera chigadzirwa kubva kuMutengesi T (A: TIANGEN, B: Mutengesi T. Sanganisa iyo template ye5 yekumanikidza gradients, uye enzanisa nemaCt values (C). Mhedzisiro yacho inoratidza kuti SuperReal PreMix Ruvara (SYBR Green) ine hukuru hwepamusoro, hushe uye kugadzikana.Kuyedza kwakamhanyisa paBio-Rad CFX96. |
Kubvira payakagadzwa, fekitori yedu yanga ichigadzira zvigadzirwa zvekutanga zvepasirese nekutevedzera mutemo
yemhando yekutanga. Zvigadzirwa zvedu zvakawana mukurumbira wakanaka muindasitiri uye yakakosha kuvimba pakati pevatengi vatsva nevakuru ..