Tarenda qPCR PreMix (SYBR Green)

Kwakanaka kupokana nekusachena kupindira uye nekukurumidza kuwanda kwematemplate akaomarara.

Ichi chigadzirwa chakakosha reagent yeiyo Chaiyo-Nguva PCR uchishandisa SYBR Green I chimeric fluorescence. Iyo 2 × Tarenda qPCR PreMix inotora antibody-yakagadziridzwa Anti Taq DNA polymerase, uye yakasarudzika qPCR Buffer system inogona kuve nechokwadi kuti chigadzirwa chinoita zvine hunyanzvi uye nekukurumidza qPCR kuita pane ese Real-Nguva PCR zviridzwa. Iyo qPCR yekuita nguva inogona kupfupiswa ne50%. Uye zvakare, kuwedzerwa kweH-Competitor factor uye EP chikamu muBuffer kunoita kuti chigadzirwa chive nemhando dzakasiyana dzemuenzaniso wepasirese. Iyo ine yakanaka kwazvo kushanda kune matemplate ane akaoma epamberi zvivakwa, matemplate ane akawanda PCR inhibitor masara, marefu fragment kukwidziridzwa, nezvimwe.
Chigadzirwa chine zvakare hunhu hwekukwirisa mwero, kukwirisa kwakanyanya, huwandu hwakavimbika, nezvimwe, uye inogona kuwana mhedzisiro nekukurumidza isina kukanganisa PCR mhedzisiro.

Cat. Aihwa Kurongedza Saizi
4992882 20 ×l × 125 rxn
4992775 20 ×l × 500 rxn
4992866 20 ×l × 5000 rxn

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

Chigadzirwa Mateki

Zvimiro

Antib Zvakanyanya kujeka antibody yakashandurwa polymerase inogona kupfupisa nguva yekuita nehafu.
■ Iyo H-Competitor inokwikwidza nehydrogen bond, iyo inovimbisa yakakwirira kukwidziridzwa kugona kwema templates ane yakakwira GC zvemukati, yakaoma sekondari chimiro uye marefu matemplate.
■ EP chinhu chinodzikamisa PCR system, zvinonyatso chengetedza enzyme zviitiko uye zvinodzivirira kupindira kweakasiyana maanhibitors.
■ Riri pachena risina chubhu rinotsiva chubhu tsvuku, kuitira kuti mari yasara ioneke.

Zvishandiso

Iyo inokodzera kuongorora kutaura, nucleic acid kuona uye mamwe marudzi ekuyedza pane akasiyana chaiwo nguva PCR zviridzwa neSYBR Green nzira, kunyanya kune yakakwira GC, yakaoma sekondari dhizaini, yakasviba yakasara yakasara uye yakareba template quantification.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)


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    Experimental Example

    Yevanhu UH11 gene (GC: 73.4%) yakaverengerwa neTIANGEN Talent qPCR PreMix (kuruboshwe) uye chakakosha chigadzirwa kubva kuMutengesi A (wepakati) uye B (kurudyi). Mhedzisiro yacho inoratidza kuti ichienzaniswa nevamwe vatengesi, Talent qPCR PreMix ine yakajeka makwikwi emakorosita mashanu emakoradhi gradients, yakaderera Ct kukosha, yepamusoro fluorescence kukosha, uye imwechete melting curve peak, inoratidza Talent qPCR PreMix ine yakanaka inochinjika uye yekukwenenzvera kugona kune akakwirira maGC matemplate.

    Nyora yako meseji pano uye uitumire kwatiri