HRM Kuongorora Kit (EvaGreen)

Zvimiro

■ Kugadziriswa kwepamusoro: Adopt EvaGreen akazara madhayi, ane resolution yepamusoro yekunyungudika curve mune yakazara nyika uye inogona kusiyanisa kusiana kweimwe base.
High Kukwirira kwakanangana: Shandisa antibody yakagadziridzwa hotstart DNA polymerase kudzikisira isina-kusimbisa kukwidziridzwa uye kugadzirisa hunhu.
■ Kugadzikana kwepamusoro: Iyo yakanyatsogadzirisa Buffer system inowedzera kugadzikana kwekunyungudika curve uye kugadzirisa kuvimbika kwemhedzisiro.
■ ROX kururamisa: ROX dhayi yakaiswa zvakasiyana, izvo zvinoshanduka kushandisa uye zvine mhedzisiro yakajeka.

Tsanangudzo

Type: Antibody yakagadziridzwa Taq DNA polymerase, EvaGreen.
Mafomu: Inozivikanwa SNP typing; Kuzivikanwa SNP kuongorora; Hazvizivikanwe mutant genes kuongorora; Methylation PCR kuongorora.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

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Genomic DNA yakabviswa kubva ku100 μl yemuenzaniso yeropa revanhu uchishandisa TIANamp Ropa reDNA Kit. 50 ng DNA yakaremerwa kwenguva chaiyo yekuonekwa kwePCR. Sekureva kweNCBI SNP raibhurari, inozivikanwa SNP yeFEN1 gene (RS 174538) inosarudzwa uye inoonekwa ichishandisa Roche LightCycle480. Mhedzisiro yacho ndeinotevera:

Mhedzisiro yacho inoratidza kuti HRM Analysis Kit ndiyo yakanaka SNP locus ongororo nzira ine yakanyanya kudzokorora kwekunyungudika curve yeiyo imwechete genotype, yakajeka resolution yeakasiyana genotypes uye pasina zvisizvo kutonga.

SNP ruzivo: …… .. CGGAAGAACACGTCG [A / G]

Primer: Ye 108 bp chidimbu (FEN1_F: CCTCAACGCTCTCACCATTTTG; FEN1_R: GGCACTTCCTTTTCCGGTTGTG)