■ Mamiriro ekuchengetedza: Iyi kit inokwanisa kuchengetwa kwevhiki rimwe kutembiricha yemukati, zuva rimwe pa37 ℃, uye mwedzi unokwana 1 pa4 ℃. Pamasampuli esuruvara, nyudza pa4 ℃ husiku, uye wotamisira ku -20 ℃ kana -80 ℃ yekuchengetedza kwenguva refu.
■ Kudzokororwa kwechando nekunyungudika: Nyama yakaomeswa nechando pa -20 ℃ kana -80 ℃ inogona kuomeswa nechando kwenguva makumi maviri isingakanganise mhando yeRNA yekubvisa.
■ Maodzanyemba ekushandisa: Mushure mekubvisa muyenzaniso kubva kuRNAstore Reagent, yakazara RNA inogona kubviswa neTIANGEN's TRNzol, RNAprep Yakachena, RNAsimple reagents uye makiti.
■ RNAstore inokodzera chete masampuli manyowani matsva.
■ RNAstore haina kukodzera masampuli eturu yemiti.
■ Chiyero cheuwandu hwemashutu esampukisi uye RNAstore Reagent inofanira kunge ingangoita 1: 10 (semuenzaniso, 100 mg tishu, inosvika 1 ml yeRNAstore inodiwa).
The Ukobvu hwedivi rega remuenzaniso haufanirwe kudarika 0.5 cm kuona kuti RNAstore inogona kupinda nekukurumidza munyama.
Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)
Zvinyorwa: 15 mg Rat chiropa tishu Maitiro: 0.5 g rat chiropa zvinyama (zvakachengetwa muRNAstore Reagent) zvakachengetwa pa37 ℃, tembiricha yemukati uye 4 ℃ zvichiteerana. Iyo yakazara RNA kubva ku15 mg rat rat chiropa masuru sampuli akachengetwa pane akasiyana tembiricha aive akasarudzika achishandisa iyo TRNzol Reagent (Cat. No. 4992730). Mhedzisiro: Ndokumbira kuti uone iri pamusoro agarose gel electrophoresis pikicha. 2-4 μl e100 μl eluates akatakurwa pamugwagwa. C (yakanaka kudzora): tishu sampuro yakachengetwa yakanangana pa -80 ℃. Iyo electrophoresis yakaitwa pa6 V / cm kwemaminitsi makumi matatu pa1% agarose. |
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Zvinyorwa: 15 mg Rat chiropa tishu Maitiro: 0.5 g rat chiropa zvinyama (zvakachengetwa muRNAstore Reagent) zvakaomeswa zve5, 10, 15 uye makumi maviri nguva zvichiteerana. Iyo yakazara RNA kubva ku15 mg rat rat chiropa masuru sampuli yakaomeswa-nyungudika kwenguva dzakasiyana dzakasarudzika uchishandisa iyo TRNzol Reagent (Cat. Kwete. 4992730). Mhedzisiro: Ndokumbira kuti uone iri pamusoro agarose gel electrophoresis pikicha. 2-4 μl e100 μl eluates akatakurwa pamugwagwa. C (yakanaka kudzora): tishu sampuro yakachengetwa yakanangana pa -80 ℃. 5, 10, 15, 20: iyo nguva yechando-yakanyungudutswa yemasampuli. Iyo electrophoresis yakaitwa pa6 V / cm kwemaminitsi makumi matatu pa1% agarose. |
Kubvira payakagadzwa, fekitori yedu yanga ichigadzira zvigadzirwa zvekutanga zvepasirese nekutevedzera mutemo
yemhando yekutanga. Zvigadzirwa zvedu zvakawana mukurumbira wakanaka muindasitiri uye yakakosha kuvimba pakati pevatengi vatsva nevakuru ..