RNAstore Reagent

Isiri-kutonhora reagent yekudzivirira kutendeka kwemuenzaniso RNA.

Iyo RNAstore Reagent imvura, isina-chepfu inochengetedza reagent. Inokurumidza kupinda mumaseru emaviri uye inodzivirira masero asina-chando kubva kuRNA in situ nekunyatsodzivirira chiitiko cheRNase, ichiita kuti ive inokodzera kuongorora matissue gene expression profiling.
Zvekuchengetera mitezo yemashuga, munyama unogona kukurumidza kunyudzwa muRNAstore yekuchengetera kudzivirira kudzikiswa kweRNA, kuitira kuti muyenzaniso usafanirwe kugadziriswa nekukasira kana nechando muinitrogen yemvura.
RNAstore Reagent inogona kushandiswa zvakanyanya mumhando dzakasiyana dzema vertebrate, kusanganisira huropi, moyo, itsvo, spleen, chiropa, mapapu uye thymus.

Cat. Aihwa Kurongedza Saizi
4992727 100 ml

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

Chigadzirwa Mateki

Zvimiro

■ Mamiriro ekuchengetedza: Iyi kit inokwanisa kuchengetwa kwevhiki rimwe kutembiricha yemukati, zuva rimwe pa37 ℃, uye mwedzi unokwana 1 pa4 ℃. Pamasampuli esuruvara, nyudza pa4 ℃ husiku, uye wotamisira ku -20 ℃ kana -80 ℃ yekuchengetedza kwenguva refu.
■ Kudzokororwa kwechando nekunyungudika: Nyama yakaomeswa nechando pa -20 ℃ kana -80 ℃ inogona kuomeswa nechando kwenguva makumi maviri isingakanganise mhando yeRNA yekubvisa.
■ Maodzanyemba ekushandisa: Mushure mekubvisa muyenzaniso kubva kuRNAstore Reagent, yakazara RNA inogona kubviswa neTIANGEN's TRNzol, RNAprep Yakachena, RNAsimple reagents uye makiti.

Zvakakosha Zvinyorwa

■ RNAstore inokodzera chete masampuli manyowani matsva.
■ RNAstore haina kukodzera masampuli eturu yemiti.
■ Chiyero cheuwandu hwemashutu esampukisi uye RNAstore Reagent inofanira kunge ingangoita 1: 10 (semuenzaniso, 100 mg tishu, inosvika 1 ml yeRNAstore inodiwa).
The Ukobvu hwedivi rega remuenzaniso haufanirwe kudarika 0.5 cm kuona kuti RNAstore inogona kupinda nekukurumidza munyama.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)


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    Experimental Example Zvinyorwa: 15 mg Rat chiropa tishu
    Maitiro: 0.5 g rat chiropa zvinyama (zvakachengetwa muRNAstore Reagent) zvakachengetwa pa37 ℃, tembiricha yemukati uye 4 ℃ zvichiteerana. Iyo yakazara RNA kubva ku15 mg rat rat chiropa masuru sampuli akachengetwa pane akasiyana tembiricha aive akasarudzika achishandisa iyo TRNzol Reagent (Cat. No. 4992730).
    Mhedzisiro: Ndokumbira kuti uone iri pamusoro agarose gel electrophoresis pikicha.
    2-4 μl e100 μl eluates akatakurwa pamugwagwa.
    C (yakanaka kudzora): tishu sampuro yakachengetwa yakanangana pa -80 ℃.
    Iyo electrophoresis yakaitwa pa6 V / cm kwemaminitsi makumi matatu pa1% agarose.
    Experimental Example Zvinyorwa: 15 mg Rat chiropa tishu
    Maitiro: 0.5 g rat chiropa zvinyama (zvakachengetwa muRNAstore Reagent) zvakaomeswa zve5, 10, 15 uye makumi maviri nguva zvichiteerana. Iyo yakazara RNA kubva ku15 mg rat rat chiropa masuru sampuli yakaomeswa-nyungudika kwenguva dzakasiyana dzakasarudzika uchishandisa iyo TRNzol Reagent (Cat. Kwete. 4992730).
    Mhedzisiro: Ndokumbira kuti uone iri pamusoro agarose gel electrophoresis pikicha. 2-4 μl e100 μl eluates akatakurwa pamugwagwa.
    C (yakanaka kudzora): tishu sampuro yakachengetwa yakanangana pa -80 ℃.
    5, 10, 15, 20: iyo nguva yechando-yakanyungudutswa yemasampuli.
    Iyo electrophoresis yakaitwa pa6 V / cm kwemaminitsi makumi matatu pa1% agarose.
    Nyora yako meseji pano uye uitumire kwatiri