RNAclean Kit

Zvekunatsa uye kupora kweRNA.

Iyo RNAclean Kit inoshandisa yakasarudzika centrifugal adsorption ikholamu kuti inyatsoita uye nekunyatso kusunga RNA kune silica gel membranes pasi pemunyu mamiriro, ichikurisa kubviswa kweprotein, inorganic salts uye zvakasiyana siyana zvakasviba zvakasviba. Iyi kit inogona kushandiswa kuchenesa kusvika ku20 μg RNA.
Ichi kit chakashandiswa kuchenesa uye kupora RNA kubva kune enzyme reaction mhinduro (senge DNase kurapwa, kurapwa kweprotease, RNA kunyora, nezvimwewo), uye inogona zvakare kushandiswa kuchenesa RNA yakawanikwa nedzimwe nzira.
Iyo yakanatswa yakazara RNA haina mapuroteni kusvibiswa, uye inogona kushandiswa kuNorth blot, dot blot, miRNA kuburitsa, cDNA synthesis, primer kuwedzera, kusiyanisa kuratidza, nezvimwe.

Cat. Aihwa Kurongedza Saizi:
4992728 20 preps

Chigadzirwa Chigadzirwa

Kufamba kwebasa

MIBVUNZO

Chigadzirwa Mateki

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)


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    Workflow

    Mubvunzo: Column blockage

    A-1 Cell lysis kana homogenization haina kukwana

    - Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.

    A-2 Mhando yemuenzaniso yakanyanya kukura

    --Deredza huwandu hwemuenzaniso wakashandiswa kana kuwedzera huwandu hwerisiti bhaisikopo.

    Mubvunzo: Yakaderera RNA goho

    A-1 Isina kukwana sero lysis kana homogenization

    - Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.

    A-2 Mhando yemuenzaniso yakanyanya kukura

    - Ndokumbira utarise kunonyanyisa kugadzira chinzvimbo.

    A-3 RNA haina kukwidziridzwa zvizere kubva pambiru

    - Mushure mekuwedzera RNase-Yemahara mvura, siya iyo kwemaminetsi mashoma pamberi pe centrifuging.

    A-4 Ethanol iri mudenga

    - Mushure mekuchenesa, centrifuge zvakare uye bvisa iyo yekugezera bhafa zvakanyanya sezvinobvira.

    A-5 Cell tsika yepakati haina kubviswa zvachose

    - Paunenge uchiunganidza maseru, ndapota ita chokwadi chekubvisa iyo tsika yepakati zvakanyanya sezvinobvira.

    A-6 Masero akachengetwa muRNAstore haana kunyatsoita centrifuged

    - RNAstore density yakakura kupfuura avhareji sero tsika svikiro; saka simba re centrifugal rinofanira kuwedzerwa. Inokurudzirwa kucentrifuge pa3000x g.

    A-7 Yakadzika RNA zvemukati uye kuwanda mune muyenzaniso

    - Shandisa yakanaka sampuro kuona kana yakaderera-goho inokonzerwa nesampuli.

    Mubvunzo: RNA kudzikisira

    A-1 Chinyorwa hachisi chitsva

    - Nyowani nyowani inofanirwa kuchengetwa muiriidhi senitrogen nekukasira kana nekukurumidza kuiswa muRNAstore reagent kuve nechokwadi chekudzora mhedzisiro.

    A-2 Mhando yemuenzaniso yakanyanya kukura

    - Deredza sampuro yemari.

    A-3 RNase kusvibiswan

    - Kunyangwe iyo buffer yakapihwa mune kit haina RNase, zviri nyore kusvibisa RNase panguva yekubvisa uye inofanira kubatwa nehanya.

    A-4 Electrophoresis kusvibiswa

    - Tsiva iyo electrophoresis buffer uye ita shuwa kuti zvinoshandiswa uye Kurodha Buffer hazvina kusvibiswa neRNase.

    A-5 Kunyanya kurodha electrophoresis

    --Deredza huwandu hwesampuli kurodha, kurodha kwetsime rega rega hakufanirwe kupfuura 2 μg.

    Mubvunzo: DNA kusvibiswa

    A-1 Mhando yemuenzaniso yakanyanya kukura

    - Deredza sampuro yemari.

    A-2 Mimwe mienzaniso ine yakakwira DNA yezviri mukati uye inogona kurapwa neDNase.

    - Ita RNase-Yemahara DNase kurapwa kune yakawanikwa RNA mhinduro, uye iyo RNA inogona kushandiswa zvakananga kuzviedza zvinotevera mushure mekurapwa, kana inogona kuvhenekerwa neRNA yekuchenesa makiti.

    Mubvunzo: Ungabvisa sei RNase kubva pane zvekuyedza zvinoshandiswa nemagirazi?

    Kune magirazi egirazi, akabikwa pa150 ° C kwe4 h. Pamidziyo yepurasitiki, yakanyudzwa mu0.5 M NaOH kwemaminetsi gumi, ndokunyatsogezwa nemvura isina RNase uye wozadza kuti ubvise zvachose RNase. Iwo ma reagents kana mhinduro dzinoshandiswa mukuyedza, kunyanya mvura, dzinofanirwa kunge dzisina RNase. Shandisa RNase-yemahara mvura kune ese reagent gadziriro (wedzera mvura kune yakachena girazi bhodhoro, wedzera DEPC kune yekupedzisira fungidziro ye0.1% (V / V), zunza husiku uye autoclave).

    Nyora yako meseji pano uye uitumire kwatiri