RNAprep Yakachena Tissue Kit

Yekuchenesa inosvika ku100 μg yakazara RNA kubva kumaturu emhuka.

Iyo RNAprep Yakachena Tissue Kit inopa inokurumidza, yakapusa, uye inodhura-nzira nzira yekuchenesa yakazara RNA kubva kumhuka dzemhuka nekushandisa inoshanda spin ikholamu uye yakasarudzika buffer system. Iyo kit inosanganisira RNase-Mahara anotenderera makoramu CR3 ekuchenesa emhando yepamusoro RNA nekushandisa silica-membrane tekinoroji. Yakakwira-mhando yakazara RNA inogona kuwanikwa mumaminetsi makumi mana- makumi mashanu nehutsanana-hwakawanda uye haina mapuroteni uye genomic DNA kusvibiswa.

Cat. Aihwa	Kurongedza Saizi
4992236	50 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvimiro

■ Zvigadziriso zvakagadzirirwa zvinyama zvemhuka zvinoita kuti maitiro acho ave nyore uye nyore.
Uni Yakasarudzika DNase I inoderedza genomic DNA kusvibiswa.
■ Iyo yakachena-kuchena uye yakagadzirira-kushandisa RNA inokodzera kune inoyerera yekudzika mafomu.
No Hapana phenol / chloroform extraction, hapana LiCl uye ethanol precipitation, uye hapana CsCl gradients centrifugation inodiwa, izvo zvinoita kuti nzira yacho ive yakachengeteka uye yakavimbika.

Zvishandiso

■ RT-PCR.
■ Northern Blot, Dot Blot.
■ Real-Nguva PCR.
■ Chip kuongorora.
■ PolyA Kuongorora, in vitro dudziro, RNase yekudzivirira ongororo uye mamorekuru kuumbana.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: RNAprep Kwakachena Cell / Bhakitiriya Kit

Zvadaro: RNAprep Yakachena FFPE Kit

Zvinyorwa: 20 mg embryo (mazuva gumi nematatu), 15 mg itsvo, 10 mg chiropa, 15 mg spleen, 10 mg thymus, 20 mg mapapu
Maitiro: Yakazara RNA kubva kwakasiyana maturu sampuli yegonzo yakanatswa uchishandisa iyo RNAprep Yakachena Tissue Kit.
Mhedzisiro: Iyo agarose gel electrophoresis pikicha inoratidzwa pamusoro. 2-4 μl e100 μl eluates akatakurwa pamugwagwa.
M: TIANGEN DNA Mucherechedzo III;
Lane 1-2: Embryo (mazuva gumi nematatu); Nzira 3: Itsvo; Lane 4-6: Chiropa; Nzira 7: Spleen; Lane 8: Thymus; Nzira 9: Mapapu.
Iyo electrophoresis yakaitwa pa6 V / cm kwemaminitsi makumi matatu pa1% agarose gel ..

Mubvunzo: Column blockage

A-1 Cell lysis kana homogenization haina kukwana

- Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.

A-2 Mhando yemuenzaniso yakanyanya kukura

--Deredza huwandu hwemuenzaniso wakashandiswa kana kuwedzera huwandu hwerisiti bhaisikopo.

Mubvunzo: Yakaderera RNA goho

A-1 Isina kukwana sero lysis kana homogenization

- Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.

A-2 Mhando yemuenzaniso yakanyanya kukura

- Ndokumbira utarise kunonyanyisa kugadzira chinzvimbo.

A-3 RNA haina kukwidziridzwa zvizere kubva pambiru

- Mushure mekuwedzera RNase-Yemahara mvura, siya iyo kwemaminetsi mashoma pamberi pe centrifuging.

A-4 Ethanol iri mudenga

- Mushure mekuchenesa, centrifuge zvakare uye bvisa iyo yekugezera bhafa zvakanyanya sezvinobvira.

A-5 Cell tsika yepakati haina kubviswa zvachose

- Paunenge uchiunganidza maseru, ndapota ita chokwadi chekubvisa iyo tsika yepakati zvakanyanya sezvinobvira.

A-6 Masero akachengetwa muRNAstore haana kunyatsoita centrifuged

- RNAstore density yakakura kupfuura avhareji sero tsika svikiro; saka simba re centrifugal rinofanira kuwedzerwa. Inokurudzirwa kucentrifuge pa3000x g.

A-7 Yakadzika RNA zvemukati uye kuwanda mune muyenzaniso

- Shandisa yakanaka sampuro kuona kana yakaderera-goho inokonzerwa nesampuli.

Mubvunzo: RNA kudzikisira

A-1 Chinyorwa hachisi chitsva

- Nyowani nyowani inofanirwa kuchengetwa muiriidhi senitrogen nekukasira kana nekukurumidza kuiswa muRNAstore reagent kuve nechokwadi chekudzora mhedzisiro.

A-2 Mhando yemuenzaniso yakanyanya kukura

- Deredza sampuro yemari.

A-3 RNase kusvibiswan

- Kunyangwe iyo buffer yakapihwa mune kit haina RNase, zviri nyore kusvibisa RNase panguva yekubvisa uye inofanira kubatwa nehanya.

A-4 Electrophoresis kusvibiswa

- Tsiva iyo electrophoresis buffer uye ita shuwa kuti zvinoshandiswa uye Kurodha Buffer hazvina kusvibiswa neRNase.

A-5 Kunyanya kurodha electrophoresis

--Deredza huwandu hwesampuli kurodha, kurodha kwetsime rega rega hakufanirwe kupfuura 2 μg.

Mubvunzo: DNA kusvibiswa

A-1 Mhando yemuenzaniso yakanyanya kukura

- Deredza sampuro yemari.

A-2 Mimwe mienzaniso ine yakakwira DNA yezviri mukati uye inogona kurapwa neDNase.

- Ita RNase-Yemahara DNase kurapwa kune yakawanikwa RNA mhinduro, uye iyo RNA inogona kushandiswa zvakananga kuzviedza zvinotevera mushure mekurapwa, kana inogona kuvhenekerwa neRNA yekuchenesa makiti.

Mubvunzo: Ungabvisa sei RNase kubva pane zvekuyedza zvinoshandiswa nemagirazi?

Kune magirazi egirazi, akabikwa pa150 ° C kwe4 h. Pamidziyo yepurasitiki, yakanyudzwa mu0.5 M NaOH kwemaminetsi gumi, ndokunyatsogezwa nemvura isina RNase uye wozadza kuti ubvise zvachose RNase. Iwo ma reagents kana mhinduro dzinoshandiswa mukuyedza, kunyanya mvura, dzinofanirwa kunge dzisina RNase. Shandisa RNase-yemahara mvura kune ese reagent gadziriro (wedzera mvura kune yakachena girazi bhodhoro, wedzera DEPC kune yekupedzisira fungidziro ye0.1% (V / V), zunza husiku uye autoclave).

Nyora yako meseji pano uye uitumire kwatiri