RNA Nyore Inokurumidza Kudyara Tissue Kit
Zvimiro
■ Zvakareruka uye zvinokurumidza: Yakazara RNA kubviswa kubva kumasampuli emiti inogona kupedzwa mukati memaminitsi makumi matatu.
■ Kuenderana kwakasimba: Iyi kititi haina kukodzera chete kune dzakajairika dzedanda nemashizha emashizha, asiwo kune polysaccharide / polyphenol-yakapfuma sampuli.
■ Yakachengeteka uye yakaderera chepfu: Reagents ane chepfu akadai se β-mercaptoethanol, DTT, chloroform, phenol hazvidiwe.
Zvishandiso
■ Inokodzera mashandiro epasi, kusanganisira RT-PCR, RT-qPCR, chip hybridization, Northern Blot, Dot Blot, PolyA kuongorora, in vitro translation, RNase kudzivirira ongororo uye mamorekuru kuumbana, nezvimwe.
Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)
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Iyo yakazara RNA yemakumi matanhatu nemashanu emashizha emashizha (mashizha evhavha, hibiscus mashizha, mashizha egorosi, mashizha emupunga, mashizha emuchero, Prunus cerasifera mashizha, mashizha emuonde, mashizha ezuva nezuva, Kerria japonica mashizha), 150 mg fungus sampuli (Lentinus edodes) uye 200 mg petal sampuli (masikati-maruva peturu) akaburitswa achishandisa RNA Easy Fast Plant Tissue Kit. |
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Agilent Bioanalyzer 2100 ongororo mhedzisiro yeRNA yakazara kubva kuZuva-maruva peturu. |
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RNA yakatorwa kubva kumasampuro akasiyana akanyorwa mutafura uchishandisa RNA Easy Fast Plant Tissue Kit. Elution vhoriyamu: 50 μl; Kuisa vhoriyamu: 2 μl. Iyo electrophoresis yakaitwa pa6 V / cm kwemaminetsi gumi nemashanu pane 1% agarose. |
A-1 Cell lysis kana homogenization haina kukwana
- Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.
A-2 Mhando yemuenzaniso yakanyanya kukura
--Deredza huwandu hwemuenzaniso wakashandiswa kana kuwedzera huwandu hwerisiti bhaisikopo.
A-1 Isina kukwana sero lysis kana homogenization
- Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.
A-2 Mhando yemuenzaniso yakanyanya kukura
- Ndokumbira utarise kunonyanyisa kugadzira chinzvimbo.
A-3 RNA haina kukwidziridzwa zvizere kubva pambiru
- Mushure mekuwedzera RNase-Yemahara mvura, siya iyo kwemaminetsi mashoma pamberi pe centrifuging.
A-4 Ethanol iri mudenga
- Mushure mekuchenesa, centrifuge zvakare uye bvisa iyo yekugezera bhafa zvakanyanya sezvinobvira.
A-5 Cell tsika yepakati haina kubviswa zvachose
- Paunenge uchiunganidza maseru, ndapota ita chokwadi chekubvisa iyo tsika yepakati zvakanyanya sezvinobvira.
A-6 Masero akachengetwa muRNAstore haana kunyatsoita centrifuged
- RNAstore density yakakura kupfuura avhareji sero tsika svikiro; saka simba re centrifugal rinofanira kuwedzerwa. Inokurudzirwa kucentrifuge pa3000x g.
A-7 Yakadzika RNA zvemukati uye kuwanda mune muyenzaniso
- Shandisa yakanaka sampuro kuona kana yakaderera-goho inokonzerwa nesampuli.
A-1 Chinyorwa hachisi chitsva
- Nyowani nyowani inofanirwa kuchengetwa muiriidhi senitrogen nekukasira kana nekukurumidza kuiswa muRNAstore reagent kuve nechokwadi chekudzora mhedzisiro.
A-2 Mhando yemuenzaniso yakanyanya kukura
- Deredza sampuro yemari.
A-3 RNase kusvibiswan
- Kunyangwe iyo buffer yakapihwa mune kit haina RNase, zviri nyore kusvibisa RNase panguva yekubvisa uye inofanira kubatwa nehanya.
A-4 Electrophoresis kusvibiswa
- Tsiva iyo electrophoresis buffer uye ita shuwa kuti zvinoshandiswa uye Kurodha Buffer hazvina kusvibiswa neRNase.
A-5 Kunyanya kurodha electrophoresis
--Deredza huwandu hwesampuli kurodha, kurodha kwetsime rega rega hakufanirwe kupfuura 2 μg.
A-1 Mhando yemuenzaniso yakanyanya kukura
- Deredza sampuro yemari.
A-2 Mimwe mienzaniso ine yakakwira DNA yezviri mukati uye inogona kurapwa neDNase.
- Ita RNase-Yemahara DNase kurapwa kune yakawanikwa RNA mhinduro, uye iyo RNA inogona kushandiswa zvakananga kuzviedza zvinotevera mushure mekurapwa, kana inogona kuvhenekerwa neRNA yekuchenesa makiti.
Kune magirazi egirazi, akabikwa pa150 ° C kwe4 h. Pamidziyo yepurasitiki, yakanyudzwa mu0.5 M NaOH kwemaminetsi gumi, ndokunyatsogezwa nemvura isina RNase uye wozadza kuti ubvise zvachose RNase. Iwo ma reagents kana mhinduro dzinoshandiswa mukuyedza, kunyanya mvura, dzinofanirwa kunge dzisina RNase. Shandisa RNase-yemahara mvura kune ese reagent gadziriro (wedzera mvura kune yakachena girazi bhodhoro, wedzera DEPC kune yekupedzisira fungidziro ye0.1% (V / V), zunza husiku uye autoclave).
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