Magnetic Serum / Plasma DNA Maxi Kit

Inokodzera kuburitsa DNA kubva pa2-5 ml yeserum / plasma ine yakakwira throughput.

Iyo kitsi inotora magineti mhete ine yakasarudzika yekuparadzanisa basa uye yakasarudzika buffer system yekuparadzanisa nekuchenesa yemhando yepamusoro yemahara DNA kubva kumasampuli senge serum uye plasma ine huwandu hwe2-5 ml. Yakasarudzika yakaiswa magineti mabheji ane hukama hwakasimba hweiyo nucleic acid pasi pemamwe mamiriro. Kana mamiriro acho achinge achinja, iwo magineti marozari anoburitsa adsorbed nucleic acid, zvichizadzisa chinangwa chekukurumidza kupatsanura uye kuchenesa kweiyo nucleic acid. Maitiro ese akachengeteka uye ari nyore. Iyo yakaburitswa yemahara nucleic acid ine yakakwira goho, yakakwira kuchena, yakagadzikana uye yakavimbika mhando, uye inonyanya kukodzera otomatiki kuburitsa kwepamusoro kuburitsa nzvimbo dzekushandira.

Cat. Aihwa	Kurongedza Saizi
4992413	2 ml × 50 preps
4992414	2 ml × 200 preps
4992917	2 ml × 1000 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvimiro

■ Kit yacho inogona kusangana nezvinodiwa zvekuburitsa manyore pamwe nekutora batch pane akasiyana mapuratifomu epamusoro-soro.
■ Zvigadzirwa zvakawanikwa nekiti zvinoenderana nezvinodiwa pakuedza kwekudzika kworwizi uye ongororo ye NGS.
■ Ichi chigadzirwa chakakodzera sarum uye masampula eplasma ane huwandu hwe2-5 ml.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: Magnetic Serum / Plasma DNA Kit

Zvadaro: Magnetic Ivhu Uye Stool DNA Kit

cfDNA yakaburitswa ne TIANGEN Magnetic Serum / Plasma DNA Maxi Kit uye chakakodzera chigadzirwa kubva kuMutengesi M kubva 2 ml serum sampuli.

Mufananidzo 1: Ita Agilent2100 kuongorora mushure meCDDNA raibhurari yekuvaka.

Tafura 1: Qubit HS: Kuunganidzwa kweDNA kunoonekwa nekunzwa kwakanyanya Qubit.

Ct Kukosha: qPCR huwandu hwemhedzisiro ine globulin primers.

Agilent 2100: Kusungwa pa300 bp chinzvimbo cheDNA raibhurari yekuvaka yakaongororwa neAgilent 2100 Chip.

Mubvunzo: Diki kana isina DNA mune iyo eluent.

A-1 Yakadzika kuwanda kwemasero kana hutachiona mune yekutanga sampuro -Pfumisa kuwanda kwemasero kana mavhairasi.

A-2 Kukwana kukosheswa kwemasampuli -Iyo mienzaniso haina kusanganiswa zvakaringana neye lysis buffer. Izvo zvinokurudzirwa kunyatso sanganisa ne pulse-vortexing kwe 1-2 nguva. —Iyo isina kukwana cell lysis inokonzerwa neicho chiitiko kuderera kweproteinase K. - Isina kukwana cell lysis kana protein degradation nekuda kwekushomeka kwekushambidza kwenguva yekugeza. Zvinokurudzirwa kutema zvidimbu zvidimbu zvidiki uye nekuwedzera nguva yekugeza kubvisa ese masara mu lysate.

A-3 Yakakwana DNA adsorption. -Hapana ethanol kana yakaderera-muzana panzvimbo pe100% ethanol yakawedzerwa isati yadzoserwa kune lysate kune spin column.

A-4 Iyo pH kukosha kwe elution buffer yakanyanya kuderera. - Chinja pH kuti ive pakati pe8.0-8.3.

Mubvunzo: DNA haiite mushe murwizi enzymatic reaction experiments.

Residual ethanol mune iyo eluent.

—Pane zvakasara zvekuwacha buffer PW mune yakasviba. Iyo ethanol inogona kubviswa ne centrifuging iyo spin ikholamu ye 3-5 min, uye nekuisa pakamuri tembiricha kana 50 ℃ incubator ye 1-2 min.

Mubvunzo: DNA kudzikisira

A-1 Muenzaniso hausi mutsva. -Tora muenzaniso wakanaka weDNA senzira yekuona kuti DNA iri musampu yanyadzisa here.

A-2 Isina kunaka pre-kurapwa. -Yakakonzerwa nekuwandisa kwemvura senitrogen kukuya, hunyoro hunowanikwa, kana huwandu hwakanyanya hwemuenzaniso.

Mubvunzo: Maitiro ekuitisa prereatment yeiyo gDNA kuburitsa?

Iwo ekufanofanirwa anofanira kusiyana pamasampuli akasiyana. Pamasampuli emiti, ita shuwa yekukuya zvakakwana muinitrogen yemvura. Kune sampuli yemhuka, homogenate kana kunyatso kukuya muinitrogen yemvura. Kune masampuli ane maseru maseru ayo akaoma kutyora, akadai seG + mabhakitiriya uye mbiriso, zvinokurudzirwa kushandisa lysozyme, lyticase kana mechina nzira kuputsa masero masvingo.

Mubvunzo: Ndeupi musiyano uripo pakati pemiti mitatu yekudyara gDNA kits 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Dyara Genomic DNA Kit inotora nzira-yakavakirwa nzira inoda chloroform yekubvisa. Inonyanya kukodzera kune akasiyana masampuli emiti, pamwe neyakaomeswa poda yakaoma. Hi-DNAsecure Plant Kit inoiswawo mumutsetse, asi pasina chikonzero che phenol / chloroform kuburitsa, ichiita kuti ive yakachengeteka uye isiri chepfu. Iyo inokodzera zvirimwa zvine yakakwirira polysaccharides uye polyphenol zvemukati. 4992709/4992710 DNAquick Plant System inotora nzira yemvura-yakavakirwa. Phenol / chloroform kuburitsa hazvidiwe futi. Maitiro ekuchenesa ari nyore uye anokurumidza pasina muganho wemuenzaniso wekutanga, saka vashandisi vanogona kugadzirisa huwandu hwacho nenzira inoenderana nezvinodiwa pakuedza. Kukura kukuru kwezvimedu zveGDNA zvinogona kuwanikwa nezvibereko zvakakura.

Ndezvipi zvibereko zvinofungidzirwa zveGDNA kubva 1 ml ropa sampuro neTIANamp Ropa reDNA Kit?

Iyo genomic DNA yakaburitswa kubva kumavhoriyamu akasiyana eropa rese revanhu neTIANamp Ropa reDNA Kit. Mhedzisiro yacho ndeinotevera. Mhedzisiro yacho yakanyorwa sereferenzi chete, iyo chaiyo mhedzisiro mhedzisiro inoenderana nemamiriro esampuli.

Mubvunzo: 4992207/4992208 uye 4992722/4992723 ingashandiswe kubvisa ropa rakagwamba DNA?

Ropa rinovhara ropa reDNA rinogona kuitwa pachishandiswa reagents yakapihwa mumakiti maviri aya nekungochinja protocol kune yakatarwa murau reropa rekuvhara ropa reDNA. Iyo yakapfava kopi yeropa clot DNA yekubvisa protocol inogona kupihwa pakukumbira.

Mubvunzo: Kana uchizora TIANamp Genomic DNA Kit, maitiro ekutyora matishu matsva museru kuturika?

Misa svumbunuro nyowani ne 1 ml PBS, yakasarudzika saline kana TE buffer. Zvakanyatso homogenize muyenzaniso ne homogenizer uye unganidza iyo inodzika pasi pezere pombi ne centrifuging. Dzinga iyo inoshamisa, uye dzorerazve iyo precipitate ne200 μl buffer GA. Iyo inotevera yekunatswa kweDNA inogona kuitwa maererano nemirairo.

Mubvunzo: Ungasarudza sei chigadzirwa cheiyo DNA kubviswa kubva muplasma, serum uye nemuviri maseru sampu?

Zvekucheneswa kweGDNA muplasma, serum uye muviri fluid sampuro, TIANamp Micro DNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kuSerum / plasma sampuro, TIANamp Virus DNA / RNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kune serum uye plasma sampuro, TIANamp Bacteria DNA Kit inokurudzirwa (lysozyme inofanirwa kuverengerwa kune yakanaka bhakitiriya). Zvematehwe sampu, Hi-Swab DNA Kit uye TIANamp Bacteria DNA Kit zvinokurudzirwa.

Mubvunzo: Ungasarudza sei makiti eiyo gDNA kubviswa kubva kune fungi sampuli?

DNAsecure Plant Kit kana DNAquick Plant System inokurudzirwa kubviswa kwefungal genome. Nezve mbiriso genome kuburitsa, TIANamp Yeast DNA Kit inokurudzirwa (lyticase inofanira kuzvigadzirira).

Cell Yemahara Dna Kubvisa Kit