TRNzol Yese Reagent

Nyowani yekuvandudza fomula yehupamhi sampuli kuchinjika.

TRNzol Universal Reagent inogona kushandiswa kupatsanura yakazara RNA kubva kumasampuli senge hutachiona, bhakitiriya, fungus, mhuka, chirimwa uye chirimwa chemuviri zvine nani lysis kugona uye kwakanyanya kunzwisisika. Iyo inochengetedza kutendeka kweRNA ichikanganisa maseru uye kuparara maseru maseru panguva yemuenzaniso homogenization. RNA yakasarudzika nechigadzirwa ichi inogona kushanda zvakananga sematemplate ekuonekwa kwekudzika kana zvimwe zvinoshandiswa.

Cat. Aihwa	Kurongedza Saizi
4992730	100 ml

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvimiro

■ Kukwirira kuchinjika: Hwande hwakawanda hwekutanga sampuro vhoriyamu, inokodzera hombe vhoriyamu sampuro kuburitsa mune imwechete maitiro.
■ Goho repamusoro: Nzira yekunaya inowedzera goho reRNA musampuro.
■ Kushandisa kwakakura: Inokodzera mienzaniso yakawanda yakasiyana senge michero yemiti nemhuka, maseru akakwenenzverwa, ropa, mvura yemuviri, nezvimwe.
Operation Kushanda nekukurumidza: Genomic DNA inogona kuwanikwa mukati meawa rimwe ..

Tsanangudzo

Type: Kunaya kunobva
Mharidzo: Utachiona, bhakitiriya, fungus, mhuka, micheka yemiti, maseru akakwenenzverwa uye mvura yemuviri.
Target: RNA
Nguva yekushanda: ~ 1 awa
Mafomu: TRNzol Universal reagent inoderedza kusvibiswa kwezvisina kuchena senge DNA nemapuroteni mune yakacheneswa yakazara RNA, uye inogona kushandiswa zvakananga kune akasiyana mamorekuru biology kuyedza senge Northern Blot, Dot Blot, PolyA kuongorora, in vitro translation, RNase kuchengetedza ongororo, cDNA raibhurari yekuvaka , RT-PCR, chaiyo nguva PCR uye yakakwira-yekupfuura kuteedzana.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: RNAprep Yakachena Micro Kit

Zvadaro: RNAprep Kwakachena Cell / Bhakitiriya Kit

Workflow

Maitiro: 30 mg rat rat chiropa zvinyama, 100 mg mupunga mashizha akaunganidzwa nekumwa senitrogen kukuya; 1 × 10⁶HepG2 maseru akagadziriswa uye 700 μl Saccharomyces Cerevisiae tsika yepakati (OD600 = 0.9) yakaunganidzwa necentrifugation. 1 ml yeTrNzol Universal Reagent kubva kuTIANGEN uye zvigadzirwa zvakafanira kubva kumutengesi L uye T zvakawedzerwa kune yega aliquot yemuenzaniso uye kubviswa kweRNA kwakaitwa zvichitevera zviga zvakapihwa nemumwe mupi. Iyo elution vhoriyamu yaive 80 μl, 50 μl, 30 μl uye 30 μl yemasampuli mana zvakateedzana. 3 μl ye eluate yakaiswa pamugwagwa.
MIII: TIANGEN Maki III;
Iyo electrophoresis yakaitwa pa6 V / cm ye30 min pa1% agarose.
Mhedzisiro: TIANGEN TRNzol Universal Reagent inogona kubvisa yakachena uye yakatendeka RNA kubva pagonzo chiropa, mashizha emupunga, maseru akakwenenzverwa uye mbiriso sampuro, ine hunyanzvi hwekuita. Iyo RNA mhando inofananidzwa kana kana yakati kurei kupfuura iya yemutengesi L uye T zvigadzirwa.

Mubvunzo: Column blockage

A-1 Cell lysis kana homogenization haina kukwana

- Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.

A-2 Mhando yemuenzaniso yakanyanya kukura

--Deredza huwandu hwemuenzaniso wakashandiswa kana kuwedzera huwandu hwerisiti bhaisikopo.

Mubvunzo: Yakaderera RNA goho

A-1 Isina kukwana sero lysis kana homogenization

- Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.

A-2 Mhando yemuenzaniso yakanyanya kukura

- Ndokumbira utarise kunonyanyisa kugadzira chinzvimbo.

A-3 RNA haina kukwidziridzwa zvizere kubva pambiru

- Mushure mekuwedzera RNase-Yemahara mvura, siya iyo kwemaminetsi mashoma pamberi pe centrifuging.

A-4 Ethanol iri mudenga

- Mushure mekuchenesa, centrifuge zvakare uye bvisa iyo yekugezera bhafa zvakanyanya sezvinobvira.

A-5 Cell tsika yepakati haina kubviswa zvachose

- Paunenge uchiunganidza maseru, ndapota ita chokwadi chekubvisa iyo tsika yepakati zvakanyanya sezvinobvira.

A-6 Masero akachengetwa muRNAstore haana kunyatsoita centrifuged

- RNAstore density yakakura kupfuura avhareji sero tsika svikiro; saka simba re centrifugal rinofanira kuwedzerwa. Inokurudzirwa kucentrifuge pa3000x g.

A-7 Yakadzika RNA zvemukati uye kuwanda mune muyenzaniso

- Shandisa yakanaka sampuro kuona kana yakaderera-goho inokonzerwa nesampuli.

Mubvunzo: RNA kudzikisira

A-1 Chinyorwa hachisi chitsva

- Nyowani nyowani inofanirwa kuchengetwa muiriidhi senitrogen nekukasira kana nekukurumidza kuiswa muRNAstore reagent kuve nechokwadi chekudzora mhedzisiro.

A-2 Mhando yemuenzaniso yakanyanya kukura

- Deredza sampuro yemari.

A-3 RNase kusvibiswan

- Kunyangwe iyo buffer yakapihwa mune kit haina RNase, zviri nyore kusvibisa RNase panguva yekubvisa uye inofanira kubatwa nehanya.

A-4 Electrophoresis kusvibiswa

- Tsiva iyo electrophoresis buffer uye ita shuwa kuti zvinoshandiswa uye Kurodha Buffer hazvina kusvibiswa neRNase.

A-5 Kunyanya kurodha electrophoresis

--Deredza huwandu hwesampuli kurodha, kurodha kwetsime rega rega hakufanirwe kupfuura 2 μg.

Mubvunzo: DNA kusvibiswa

A-1 Mhando yemuenzaniso yakanyanya kukura

- Deredza sampuro yemari.

A-2 Mimwe mienzaniso ine yakakwira DNA yezviri mukati uye inogona kurapwa neDNase.

- Ita RNase-Yemahara DNase kurapwa kune yakawanikwa RNA mhinduro, uye iyo RNA inogona kushandiswa zvakananga kuzviedza zvinotevera mushure mekurapwa, kana inogona kuvhenekerwa neRNA yekuchenesa makiti.

Mubvunzo: Ungabvisa sei RNase kubva pane zvekuyedza zvinoshandiswa nemagirazi?

Kune magirazi egirazi, akabikwa pa150 ° C kwe4 h. Pamidziyo yepurasitiki, yakanyudzwa mu0.5 M NaOH kwemaminetsi gumi, ndokunyatsogezwa nemvura isina RNase uye wozadza kuti ubvise zvachose RNase. Iwo ma reagents kana mhinduro dzinoshandiswa mukuyedza, kunyanya mvura, dzinofanirwa kunge dzisina RNase. Shandisa RNase-yemahara mvura kune ese reagent gadziriro (wedzera mvura kune yakachena girazi bhodhoro, wedzera DEPC kune yekupedzisira fungidziro ye0.1% (V / V), zunza husiku uye autoclave).

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