■ Inyore uye yakapusa: 96 sampuli dzinogona kuburitswa mumaawa 2-3.
■ Yakashongedzerwa neakasarudzika TIANRed reagent, inogona kujekesa zvakajeka mwero we lysis yemuenzaniso mune yekudzora maitiro kuti ive yakachena-yakachena plasmid DNA.
■ Kuchena kwepamusoro: Iyo yakachena-kuchena plasmid inogona kushandiswa yakanangana zvakananga mukati mekuyedza kuyedza.
Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)
Plasmid DNA yakabviswa kubva pa1 ml yeusiku hwakasimudzwa Escherichia coli (TOP10) muto (LB wepakati) uchishandisa TIANprep Rapid N96 Plasmid Kit, uye avhareji ye 8-10 μg yeiyo yakachena pBS plasmid yakawanikwa. 2 pll plasmid yakaiswa mune yega yega tsime re1% agarose gel, uye yakaonekwa neelectrophoresis. |
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Plasmid DNA yakabviswa kubva pa1 ml yeusiku hwakasimudzwa Escherichia coli (TOP10) muto (LB wepakati) uchishandisa TIANprep Rapid N96 Plasmid Kit, uye avhareji ye 8-10 μg yeiyo yakachena pBS plasmid yakawanikwa. Vector: pBS plasmid; Kushungurudzika: TOP10. Iyo plasmid yakateedzana neABI. PRISM 3730XL DNA sequencer |
Kubvira payakagadzwa, fekitori yedu yanga ichigadzira zvigadzirwa zvekutanga zvepasirese nekutevedzera mutemo
yemhando yekutanga. Zvigadzirwa zvedu zvakawana mukurumbira wakanaka muindasitiri uye yakakosha kuvimba pakati pevatengi vatsva nevakuru ..