RNAprep Yakachena Hi-Ropa Kit

Yekuchenesa kwemhando yepamusoro uye yakagadzikana yakazara RNA kubva muropa.

Iyo RNAprep Yakachena Hi-Ropa Kit inoburitsa nemazvo RNA yakazara kubva patsva izere neropa neropa ine akawanda anticoagulants kubva akasiyana marudzi. Iyo silicon matrix zvinhu zvinoshandiswa mu adsorption column ndeye yakasarudzika nyowani nyowani yakagadzirwa neTIANGEN, iyo inobudirira uye chaizvo inoshambadza RNA, uye inobvisa kusachena mapuroteni kusvika pamwero wakanyanya. Iyo RNA yakaburitswa inogona kushandiswa mukuyedza kwakasiyana-siyana kwakadzika senge RT-PCR, RT-qPCR, chip ongororo, yakakwira-nzira yekuteedzera, Northern Blot, Dot Blot, PolyA kuongorora, in vitro, RNase kuchengetedza ongororo, kuumbika kwemaMolekyuli, nezvimwe.

Cat. Aihwa	Kurongedza Saizi
4992903	50 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvimiro

■ Inokodzera ropa idzva remhando dzakasiyana, iri nyore kuvhiya.
■ Kushongedzerwa neRNase-Mahara Filtration Column CS kubvisa zvinobudirira tsvina.
■ Iyo yakanyatsogadziriswa buffer inogona kuona inoshanda uye yakagadzikana RNA kuburitsa kune dzakasiyana siyana zasi kwekuyedza.
■ Kuvhiya uye kwakavimbika mashandiro, hapana phenol / chloroform kuburitsa kunodiwa.

Zvishandiso

RT-PCR, Northern Blot, RT-qPCR, chip kuongorora, kukwirisa-kukwirisa, PolyA kuongorora, kuongorora kweRNase, in vitro translation, nezvimwe.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: RNAprep Yakachena Sima Plus Kit

Zvadaro: RNA Nyore Inokurumidza Nyama / Cell Kit

	Mufananidzo 1. RNA yakanatswa kubva ku100 μl nyowani yegonzo reropa mune dzakasiyana anticoagulants uchishandisa RNAprep Yakachena Hi-Ropa Kit. 4-6 μl ye50 μl eluates akatakurwa pamugwagwa. M: TIANGEN DNA Mucherechedzo III.
	Mufananidzo 2. RNA yakanatswa kubva ku100 μl nyowani yegonzo reropa uchishandisa RNAprep Yakachena Hi-Ropa Kit. 4-6 μl ye50 μl eluates akatakurwa pamugwagwa. M: TIANGEN DNA Mucherechedzo III.

Mubvunzo: Column blockage

A-1 Cell lysis kana homogenization haina kukwana

- Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.

A-2 Mhando yemuenzaniso yakanyanya kukura

--Deredza huwandu hwemuenzaniso wakashandiswa kana kuwedzera huwandu hwerisiti bhaisikopo.

Mubvunzo: Yakaderera RNA goho

A-1 Isina kukwana sero lysis kana homogenization

- Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.

A-2 Mhando yemuenzaniso yakanyanya kukura

- Ndokumbira utarise kunonyanyisa kugadzira chinzvimbo.

A-3 RNA haina kukwidziridzwa zvizere kubva pambiru

- Mushure mekuwedzera RNase-Yemahara mvura, siya iyo kwemaminetsi mashoma pamberi pe centrifuging.

A-4 Ethanol iri mudenga

- Mushure mekuchenesa, centrifuge zvakare uye bvisa iyo yekugezera bhafa zvakanyanya sezvinobvira.

A-5 Cell tsika yepakati haina kubviswa zvachose

- Paunenge uchiunganidza maseru, ndapota ita chokwadi chekubvisa iyo tsika yepakati zvakanyanya sezvinobvira.

A-6 Masero akachengetwa muRNAstore haana kunyatsoita centrifuged

- RNAstore density yakakura kupfuura avhareji sero tsika svikiro; saka simba re centrifugal rinofanira kuwedzerwa. Inokurudzirwa kucentrifuge pa3000x g.

A-7 Yakadzika RNA zvemukati uye kuwanda mune muyenzaniso

- Shandisa yakanaka sampuro kuona kana yakaderera-goho inokonzerwa nesampuli.

Mubvunzo: RNA kudzikisira

A-1 Chinyorwa hachisi chitsva

- Nyowani nyowani inofanirwa kuchengetwa muiriidhi senitrogen nekukasira kana nekukurumidza kuiswa muRNAstore reagent kuve nechokwadi chekudzora mhedzisiro.

A-2 Mhando yemuenzaniso yakanyanya kukura

- Deredza sampuro yemari.

A-3 RNase kusvibiswan

- Kunyangwe iyo buffer yakapihwa mune kit haina RNase, zviri nyore kusvibisa RNase panguva yekubvisa uye inofanira kubatwa nehanya.

A-4 Electrophoresis kusvibiswa

- Tsiva iyo electrophoresis buffer uye ita shuwa kuti zvinoshandiswa uye Kurodha Buffer hazvina kusvibiswa neRNase.

A-5 Kunyanya kurodha electrophoresis

--Deredza huwandu hwesampuli kurodha, kurodha kwetsime rega rega hakufanirwe kupfuura 2 μg.

Mubvunzo: DNA kusvibiswa

A-1 Mhando yemuenzaniso yakanyanya kukura

- Deredza sampuro yemari.

A-2 Mimwe mienzaniso ine yakakwira DNA yezviri mukati uye inogona kurapwa neDNase.

- Ita RNase-Yemahara DNase kurapwa kune yakawanikwa RNA mhinduro, uye iyo RNA inogona kushandiswa zvakananga kuzviedza zvinotevera mushure mekurapwa, kana inogona kuvhenekerwa neRNA yekuchenesa makiti.

Mubvunzo: Ungabvisa sei RNase kubva pane zvekuyedza zvinoshandiswa nemagirazi?

Kune magirazi egirazi, akabikwa pa150 ° C kwe4 h. Pamidziyo yepurasitiki, yakanyudzwa mu0.5 M NaOH kwemaminetsi gumi, ndokunyatsogezwa nemvura isina RNase uye wozadza kuti ubvise zvachose RNase. Iwo ma reagents kana mhinduro dzinoshandiswa mukuyedza, kunyanya mvura, dzinofanirwa kunge dzisina RNase. Shandisa RNase-yemahara mvura kune ese reagent gadziriro (wedzera mvura kune yakachena girazi bhodhoro, wedzera DEPC kune yekupedzisira fungidziro ye0.1% (V / V), zunza husiku uye autoclave).

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