FastKing Imwe Nhanho RT-qPCR kit

Imwe-nhanho Real-Nguva RT-PCR ine SYBR-Green.

FastKing Imwe Nhanho RT-qPCR Kit yakagadzirirwa Real-Nguva Imwe Nhanho RT-qPCR nekushandisa SYBR Green I. Iyo kit inogona kushandiswa mune chaiyo-nguva imwe nhanho RT-qPCR yezvinangwa zveRNA, nekudzokorodza kudhindwa uye PCR kunoitika zvakateedzana muhubhu imwechete. Naizvozvo kuvhiya kuri nyore, uye kunoderedza njodzi yekusvibiswa.

Iyo 25 × RT-PCR Enzyme Sanganisa mune ino kit iri chirevo cheTIANGEN's novel reverse transcriptase (FastKing RTase), novel antibody yakashandurwa hotstart Taq DNA polymerase uye RNase Inhibitor. Iyo FastKing RTase iri mhando nyowani yemamorekuru akagadziridzwa reverse transcriptase. Iine yakanyanya kuwedzerwa hydrophobic motif, ine yakasimba RNA kuwirirana uye kupisa kwemafuta. Iyo FastKing RTase yakavandudza kudzoreredza kunyorwa kugona uye kugona kuwedzera iyo RNA template ine yakaoma yechipiri chimiro. Rudzi rutsva rwe hotstartTaq DNA polymerase ine basa rakanakisa inoshandiswa muPCR maitiro, zvichiita kuti PCR itarise mushure mekunyorwa kwechinyorwa kuve nehukama hwekuwedzera uye hunyanzvi. Uye zvakare, iyo 2 × FastKing RT-qPCR Buffer (SYBR Green) mune ichi chigadzirwa inyowani yekuita system yakanyatsogadzirirwa mairi enzymes maviri ari pamusoro, anosanganisira anokosha ionic zvinhu, dNTPs, SYBR Green dhayi, imwe nhanho inosimudzira uye inosimudzira, iyo inogona kuve nechokwadi chekuti FastKing RTase uye hotstart nyowaniTaq DNA polymerase inogona kutamba chinzvimbo chinoshanda kwazvo mune yese-nhanho yekuita maitiro.

Iri kit rinopa akanaka akaenzana makakombama munzvimbo dzakasiyana siyana dzehuwandu hwenzvimbo. Iyo inogona kunyatso kuona uye nekuwongorora kuona dzakasiyana siyana dzakakwira uye dzakaderera kuwanda tarisa majini nekudzokorora kwakanaka uye kwakanyanya kuvimbika.