Yield Zvibereko zvinokurumidza uye zvakakwirira: 200 μg-1.5 mg plasmid DNA goho muawa rimwe riine huwandu hwakawanda hwechimiro chakakomberedzwa.
■ Kuchena-kwepamusoro: Yakachena-yakachena plasmid DNA inowanikwa neakasarudzika buffer system uye Spin Column CP6.
■ Kugona kutapuririsa hutanho: Inokodzera kuyedzwa kwechitapudzo cheyakawanda mitsara yemasero.
■ Wide akasiyana mafomu: Inokodzera kudzora enzyme kugaya, shanduko, kuteedzana, microinjection, gene kudzikamisa uye kuyedza kuyedza.
Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)
Yakakwira / yakaderera kopi plasmid kuburitsa Yakadzika-kopi plasmid pBR322, yakatorwa kubva ku200 ml hutachiona tsika uchishandisa EndoFree Maxi Plasmid Kit, yaive yakasarudzika mu1 ml Buffer TB, ine huwandu hwe0.6 μg / μl. Yakakwira-kopi plasmid pBS, yakatorwa kubva ku100 ml yebhakitiriya tsika ichishandisa EndoFree Maxi Plasmid Kit, yaive yakasarudzika mu1 ml Buffer TB, ine huwandu hwe1.2 μg / μl. MIV: TIANGEN DNA Mucherechedzo IV pBR322: 2 μl, pBS: 2 μl |
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pEGFP yakawanikwa neEndoFree Maxi Plasmid Kit yakaparadzaniswa zvakasiyana kuva endotoxin-isinganzwi sero mutsara 293T uye endotoxin-inonzwika sero mutsara Huh7 masero neTIANGEN TIANfect. Tsananguro yeGFP yakaonekwa mumaawa makumi maviri nemana mushure mekushandurwa. |
Kubvira payakagadzwa, fekitori yedu yanga ichigadzira zvigadzirwa zvekutanga zvepasirese nekutevedzera mutemo
yemhando yekutanga. Zvigadzirwa zvedu zvakawana mukurumbira wakanaka muindasitiri uye yakakosha kuvimba pakati pevatengi vatsva nevakuru ..