EndoFree Maxi Plasmid Kit

Yekuchenesa kweiyo endotoxin-yemahara yekuisa giredhi plasmid DNA.

Iyo EndoFree Maxi Plasmid Kit yakavakirwa pane silica membrane tekinoroji kubvisa endotoxin-isina plasmid DNA. Iyi kit inopa yakasarudzika endotoxin yekubvisa maitiro ekugadzira yepamusoro-soro plasmid DNA yekudzika mafomu. Iyo yakagadzirirwa kupatsanura plasmid DNA yeiyo yakanyanya kuchena kubva pa100-200 ml yehutachiona tsika uye inogona kuburitsa inosvika 1.5 mg yekuisa giredhi plasmid DNA.

Cat. Aihwa Kurongedza Saizi
4992194 10 preps

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

Chigadzirwa Mateki

Zvimiro

Yield Zvibereko zvinokurumidza uye zvakakwirira: 200 μg-1.5 mg plasmid DNA goho muawa rimwe riine huwandu hwakawanda hwechimiro chakakomberedzwa.
■ Kuchena-kwepamusoro: Yakachena-yakachena plasmid DNA inowanikwa neakasarudzika buffer system uye Spin Column CP6.
■ Kugona kutapuririsa hutanho: Inokodzera kuyedzwa kwechitapudzo cheyakawanda mitsara yemasero.
■ Wide akasiyana mafomu: Inokodzera kudzora enzyme kugaya, shanduko, kuteedzana, microinjection, gene kudzikamisa uye kuyedza kuyedza.

Kubereka kwePlasmid

Plasmid Yield

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)


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    Experimental Example Yakakwira / yakaderera kopi plasmid kuburitsa
    Yakadzika-kopi plasmid pBR322, yakatorwa kubva ku200 ml hutachiona tsika uchishandisa EndoFree Maxi Plasmid Kit, yaive yakasarudzika mu1 ml Buffer TB, ine huwandu hwe0.6 μg / μl. Yakakwira-kopi plasmid pBS, yakatorwa kubva ku100 ml yebhakitiriya tsika ichishandisa EndoFree Maxi Plasmid Kit, yaive yakasarudzika mu1 ml Buffer TB, ine huwandu hwe1.2 μg / μl.
    MIV: TIANGEN DNA Mucherechedzo IV
    pBR322: 2 μl, pBS: 2 μl
    Experimental Example pEGFP yakawanikwa neEndoFree Maxi Plasmid Kit yakaparadzaniswa zvakasiyana kuva endotoxin-isinganzwi sero mutsara 293T uye endotoxin-inonzwika sero mutsara Huh7 masero neTIANGEN TIANfect. Tsananguro yeGFP yakaonekwa mumaawa makumi maviri nemana mushure mekushandurwa.
    Nyora yako meseji pano uye uitumire kwatiri