TIANprep Rapid Mini Plasmid Kit Featured Image

TIANprep Inokurumidza Mini Plasmid Kit

Yekutsanya kunatswa kweplasmid DNA yemamorekuru biology giredhi nealkaline lysis maitiro.

Iyo TIANprep Rapid Mini Plasmid Kit yakagadziridzwa kubva kuchinyakare alkaline lysis tekinoroji, iyo yepamusoro-soro plasmid DNA inogona kucheneswa mukati memaminitsi masere. Iyo nyowani lysis buffer inobvumira kushambadzira kweDNA pane silica membrane pamberi peyakanyanya munyu. Izvo zvinhu zvakagadzirwa neiyo silica membrane yakasarudzika, inoshanda zvakanyanya uye yakanyanya kujeka. Iyi kiti yakagadzirirwa kuchenesa inosvika 24 μg plasmid DNA kubva kuna 1-4 ml husiku hutsika dzeE.coli. Iyo plasmid DNA yakanatswa neiyi kit inogona kuiswa mune akasiyana enguva dzose kuyedza, senge enzyme kugaya, PCR, kuteedzana, ligation, shanduko, raibhurari kuongorora, in vitro transcription uye kuisirwa kweiyo yakajairika sub-tsika maseru, nezvimwe.

Cat. Aihwa	Kurongedza Saizi
4992191	50 preps
4992192	200 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Kufamba kwebasa

Muenzaniso Wekuedza

Chigadzirwa Mateki

Zvimiro

■ Inokurumidza: Maitiro akareruka aine mashoma matanho ekuchenesa yakachena yakachena plasmid DNA mukati maminetsi masere chete.
■ Kubudirira kwepamusoro: Inogona kuchenesa inopfuura 85% plasmid DNA kubva E.coli.
■ Chinongedzo chemavara TIANRed chakapihwa kudzora nekuyeuchidza maitiro ese panguva yekuyedza kuve nechokwadi chepamusoro penzura yekuchenesa kweplasmid.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: TGuide S32 Yakagadzirwa Nucleic Acid Inobvisa

Zvadaro: EndoFree Midi Plasmid Kit

Iine yakakwira-inoshanda kurapa lysate uye yakagadziriswa buffer system, TIANprep Rapid Mini Plasmid Kit inogona kuwana zvirinani zvigadzirwa zvemhando yepamusoro uku ichichengetedza yakawanda yekushanda nguva.

Kufananidza pakati peTIANprep Rapid Mini Plasmid Kit uye Mutengesi A (yakajairika plasmid DNA yekuchenesa kit) nekuchenesa iyo imwechete plasmid panguva imwe chete.

Muenzaniso vhoriyamu: 3 ml husiku hutsika tsika E.coli (OD600 = 1.8); Elution vhoriyamu: 50 μl; Kuisa vhoriyamu: 1 μl pBS, 3 μl pET30a, 3 μl pBR322.

Iyo electrophoresis yakaitwa pa6 V / cm kwemaminitsi makumi matatu pa1% agarose gel. M: TIANGEN DNA Mucherechedzo IV.

Mhedziso: Agarose gel electrophoresis inoratidza kuti TIANprep Rapid Mini Plasmid Kit inogona kuwana gohwo rakafanana replasmid DNA seiyo neplasmid yekuchenesa kit mukati me8 min.

TIANprep Rapid Mini Plasmid Kit