TIANamp FFPE DNA Kit

Yakachena-inoshanda kuchenesa kweDNA kubva ku formalin-yakagadziriswa, parafini-yakadzikwa matishu ne xylene kurapwa.

TIANamp FFPE DNA Kit yakagadziridzwa kucheneswa kweDNA kubva kuFFPE tishu zvikamu. Iyo inoshandisa xylene kubvisa parafini, uye inopa yakasarudzika mamiriro ekudzora kweDNA kuburitsa kubva munyama chidimbu. Yakasanganiswa nesarudzo-inosunga silica-yakavakirwa membrane uye inoshanduka elution system, kit iyi inogona kukwirisa yemhando yepamusoro DNA kune diki vhoriyamu. Yakacheneswa DNA inogona kushanda zvakananga sematemplate ekuonekwa kwekudzika kana zvimwe zvinoshandiswa.

Cat. Aihwa	Kurongedza Saizi
4992524	50 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvimiro

■ Kuchena kwakanyanya: Silica membrane inobvisa tsvina zhinji.
■ Kushandisa kwakakura: Inokodzera chikamu cheparafini, bharoka reparafini, uye sampuli dzakadzika midzi.
■ Kuvhiya nekukurumidza: DNA inogona kuwanikwa mukati meawa rimwe chete.

Tsanangudzo

Type: Spin ikholamu yakavakirwa
Mharidzo: Parafini chikamu, parafini block, uye formalin-yakadzika sampuli
Target: Genomic DNA
Kutanga vhoriyamu: 5-8 zvidimbu zvezvikamu zveparafini kana 30 mg tishu muparafini kana formalin
Nguva yekushanda: ~ 1 awa
Maodzanyemba ekushandisa: PCR, SNP genotype ongororo, STR kuongorora, pharmacogenomics ongororo, NGS raibhurari yekuvaka, nezvimwe.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: TIANquick FFPE DNA Kit

Zvadaro: TIANprep N96 Plasmid Kit

Iyo genomic DNA yakaburitswa neTIANamp FFPE DNA Kit uye chakakodzera chigadzirwa kubva kuMutengesi A chakakwidziridzwa kusvika 190 bp, 330 bp, 500 bp, 650 bp uye 1300 bp zvidimbu. Mhedzisiro yacho inoratidza kuti DNA yakaburitswa ne TIANamp FFPE DNA Kit ine goho rakakwirira, kuchena kwakanyanya kunyanya kune zvidimbu zvikuru.

Kutanga zvinhu: Mouse chiropa FFPE sampuli (zvidimbu zvishanu, 10 μm / chidimbu)
Maitiro: Tevedzera rairo yechigadzirwa chimwe nechimwe.
Gel kurodha: gDNA yakasarudzika ne50 μl elution buffer uye yakawedzeredzwa kusvika 190 bp, 330 bp 500 bp, 650 bp uye 1300 bp. 5 μl ye20 μl PCR zvigadzirwa zvakaiswa munzira imwe pa1% agarose gel. Iyo electrophoresis yakaitiswa pa6 V / cm ye20 min.
M: TIANGEN Maki D2000;
T: TIANamp FFPE DNA Kit;
A: Chigadzirwa chakakodzera kubva kuMutengesi A.

Mubvunzo: Diki kana isina DNA mune iyo eluent.

A-1 Yakadzika kuwanda kwemasero kana hutachiona mune yekutanga sampuro -Pfumisa kuwanda kwemasero kana mavhairasi.

A-2 Kukwana kukosheswa kwemasampuli -Iyo mienzaniso haina kusanganiswa zvakaringana neye lysis buffer. Izvo zvinokurudzirwa kunyatso sanganisa ne pulse-vortexing kwe 1-2 nguva. —Iyo isina kukwana cell lysis inokonzerwa neicho chiitiko kuderera kweproteinase K. - Isina kukwana cell lysis kana protein degradation nekuda kwekushomeka kwekushambidza kwenguva yekugeza. Zvinokurudzirwa kutema zvidimbu zvidimbu zvidiki uye nekuwedzera nguva yekugeza kubvisa ese masara mu lysate.

A-3 Yakakwana DNA adsorption. -Hapana ethanol kana yakaderera-muzana panzvimbo pe100% ethanol yakawedzerwa isati yadzoserwa kune lysate kune spin column.

A-4 Iyo pH kukosha kwe elution buffer yakanyanya kuderera. - Chinja pH kuti ive pakati pe8.0-8.3.

Mubvunzo: DNA haiite mushe murwizi enzymatic reaction experiments.

Residual ethanol mune iyo eluent.

—Pane zvakasara zvekuwacha buffer PW mune yakasviba. Iyo ethanol inogona kubviswa ne centrifuging iyo spin ikholamu ye 3-5 min, uye nekuisa pakamuri tembiricha kana 50 ℃ incubator ye 1-2 min.

Mubvunzo: DNA kudzikisira

A-1 Muenzaniso hausi mutsva. -Tora muenzaniso wakanaka weDNA senzira yekuona kuti DNA iri musampu yanyadzisa here.

A-2 Isina kunaka pre-kurapwa. -Yakakonzerwa nekuwandisa kwemvura senitrogen kukuya, hunyoro hunowanikwa, kana huwandu hwakanyanya hwemuenzaniso.

Mubvunzo: Maitiro ekuitisa prereatment yeiyo gDNA kuburitsa?

Iwo ekufanofanirwa anofanira kusiyana pamasampuli akasiyana. Pamasampuli emiti, ita shuwa yekukuya zvakakwana muinitrogen yemvura. Kune sampuli yemhuka, homogenate kana kunyatso kukuya muinitrogen yemvura. Kune masampuli ane maseru maseru ayo akaoma kutyora, akadai seG + mabhakitiriya uye mbiriso, zvinokurudzirwa kushandisa lysozyme, lyticase kana mechina nzira kuputsa masero masvingo.

Mubvunzo: Ndeupi musiyano uripo pakati pemiti mitatu yekudyara gDNA kits 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Dyara Genomic DNA Kit inotora nzira-yakavakirwa nzira inoda chloroform yekubvisa. Inonyanya kukodzera kune akasiyana masampuli emiti, pamwe neyakaomeswa poda yakaoma. Hi-DNAsecure Plant Kit inoiswawo mumutsetse, asi pasina chikonzero che phenol / chloroform kuburitsa, ichiita kuti ive yakachengeteka uye isiri chepfu. Iyo inokodzera zvirimwa zvine yakakwirira polysaccharides uye polyphenol zvemukati. 4992709/4992710 DNAquick Plant System inotora nzira yemvura-yakavakirwa. Phenol / chloroform kuburitsa hazvidiwe futi. Maitiro ekuchenesa ari nyore uye anokurumidza pasina muganho wemuenzaniso wekutanga, saka vashandisi vanogona kugadzirisa huwandu hwacho nenzira inoenderana nezvinodiwa pakuedza. Kukura kukuru kwezvimedu zveGDNA zvinogona kuwanikwa nezvibereko zvakakura.

Ndezvipi zvibereko zvinofungidzirwa zveGDNA kubva 1 ml ropa sampuro neTIANamp Ropa reDNA Kit?

Iyo genomic DNA yakaburitswa kubva kumavhoriyamu akasiyana eropa rese revanhu neTIANamp Ropa reDNA Kit. Mhedzisiro yacho ndeinotevera. Mhedzisiro yacho yakanyorwa sereferenzi chete, iyo chaiyo mhedzisiro mhedzisiro inoenderana nemamiriro esampuli.

Mubvunzo: 4992207/4992208 uye 4992722/4992723 ingashandiswe kubvisa ropa rakagwamba DNA?

Ropa rinovhara ropa reDNA rinogona kuitwa pachishandiswa reagents yakapihwa mumakiti maviri aya nekungochinja protocol kune yakatarwa murau reropa rekuvhara ropa reDNA. Iyo yakapfava kopi yeropa clot DNA yekubvisa protocol inogona kupihwa pakukumbira.

Mubvunzo: Kana uchizora TIANamp Genomic DNA Kit, maitiro ekutyora matishu matsva museru kuturika?

Misa svumbunuro nyowani ne 1 ml PBS, yakasarudzika saline kana TE buffer. Zvakanyatso homogenize muyenzaniso ne homogenizer uye unganidza iyo inodzika pasi pezere pombi ne centrifuging. Dzinga iyo inoshamisa, uye dzorerazve iyo precipitate ne200 μl buffer GA. Iyo inotevera yekunatswa kweDNA inogona kuitwa maererano nemirairo.

Mubvunzo: Ungasarudza sei chigadzirwa cheiyo DNA kubviswa kubva muplasma, serum uye nemuviri maseru sampu?

Zvekucheneswa kweGDNA muplasma, serum uye muviri fluid sampuro, TIANamp Micro DNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kuSerum / plasma sampuro, TIANamp Virus DNA / RNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kune serum uye plasma sampuro, TIANamp Bacteria DNA Kit inokurudzirwa (lysozyme inofanirwa kuverengerwa kune yakanaka bhakitiriya). Zvematehwe sampu, Hi-Swab DNA Kit uye TIANamp Bacteria DNA Kit zvinokurudzirwa.

Mubvunzo: Ungasarudza sei makiti eiyo gDNA kubviswa kubva kune fungi sampuli?

DNAsecure Plant Kit kana DNAquick Plant System inokurudzirwa kubviswa kwefungal genome. Nezve mbiriso genome kuburitsa, TIANamp Yeast DNA Kit inokurudzirwa (lyticase inofanira kuzvigadzirira).

Cell Yemahara Dna Kubvisa Kit