TIANamp Ropa Mahara DNA Kit

Kubudiswa kwe genomic DNA kubva kumasero akaomeswa eropa maseru.

Iyo TIANamp Ropa Spots DNA Kit inotora iyo spin kholamu iyo inosunga chaizvo iyo DNA uye yakasarudzika buffer system yekubvisa genomic DNA kubva muropa mavara. Iyo silicon matrix zvinhu zvinoshandiswa musipinamu yekoramu ndeye yakasarudzika nyowani zvinhu zvakagadzirwa neTIANGEN, iyo inogona nemazvo uye yakanangana kushambadza DNA, uye kubvisa tsvina uye mamwe makemikari makemikari mumaseru kusvika zvakanyanya. Hukuru hukuru hweGDNA zvidimbu zvine kuchena kwakanyanya uye kugadzikana zvinogona kuwanikwa uchishandisa kit.

Cat. Aihwa Kurongedza Saizi
4992716 50 preps
4992717 200 preps

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvimiro

■ Kushandisa kwakakura: Iyo kititi inogona kushandiswa kutora gDNA kubva kune akasiyana eropa mavara masampuli akananga.
■ Yepamusoro mhando: Iine yakasarudzika lysis buffer system, iyo yakanatswa DNA ine yakanyanya kusangana, kuchena uye kusanganisa kwakanaka kunogona kugutsa zvirevo zvechip hybridization uye yakakwira-yekupfuura kuteedzana.
■ Inokurumidza uye isina chepfu: Iyo kit inoshandisa silica membrane adsorption tekinoroji uye haidi phenol uye chloroform. Maitiro ese ekuburitsa anogona kupedzwa mukati meawa rimwe chete.

Zvishandiso

Inokodzera akasiyana siyana ekushandisa, akadai sekudzivirira enzyme kugaya, PCR, kuvaka raibhurari, Maodzanyemba blot, chip kusanganiswa uye Yakakwira-yekupfuura kuteedzana, nezvimwe.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)


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    Iyo genomic DNA yemasero eropa eropa masampu akaburitswa achishandisa iyo TIANamp Ropa Spots DNA Kit zvinoenderana neyakajairwa protocol. Muenzaniso wekutanga huwandu: 3 zvidimbu zve3 x 3 mm reropa. 2 μl ye50 μl eluents akashandiswa sePCR template. Ita iyo PCR kukwidziridzwa kweActb (300 bp), PrP (750 bp) uye DN1.0 (1 kb) mune 20 μl PCR reaction system zvichiteerana. 5 μl yezvigadzirwa zvePCR zvakatakurwa pamugwagwa.

    Experimental Example

    Mubvunzo: Diki kana isina DNA mune iyo eluent.

    A-1 Yakadzika kuwanda kwemasero kana hutachiona mune yekutanga sampuro -Pfumisa kuwanda kwemasero kana mavhairasi.

    A-2 Kukwana kukosheswa kwemasampuli -Iyo mienzaniso haina kusanganiswa zvakaringana neye lysis buffer. Izvo zvinokurudzirwa kunyatso sanganisa ne pulse-vortexing kwe 1-2 nguva. —Iyo isina kukwana cell lysis inokonzerwa neicho chiitiko kuderera kweproteinase K. - Isina kukwana cell lysis kana protein degradation nekuda kwekushomeka kwekushambidza kwenguva yekugeza. Zvinokurudzirwa kutema zvidimbu zvidimbu zvidiki uye nekuwedzera nguva yekugeza kubvisa ese masara mu lysate.

    A-3 Yakakwana DNA adsorption. -Hapana ethanol kana yakaderera-muzana panzvimbo pe100% ethanol yakawedzerwa isati yadzoserwa kune lysate kune spin column.

    A-4 Iyo pH kukosha kwe elution buffer yakanyanya kuderera. - Chinja pH kuti ive pakati pe8.0-8.3.

    Mubvunzo: DNA haiite mushe murwizi enzymatic reaction experiments.

    Residual ethanol mune iyo eluent.

    —Pane zvakasara zvekuwacha buffer PW mune yakasviba. Iyo ethanol inogona kubviswa ne centrifuging iyo spin ikholamu ye 3-5 min, uye nekuisa pakamuri tembiricha kana 50 ℃ incubator ye 1-2 min.

    Mubvunzo: DNA kudzikisira

    A-1 Muenzaniso hausi mutsva. -Tora muenzaniso wakanaka weDNA senzira yekuona kuti DNA iri musampu yanyadzisa here.

    A-2 Isina kunaka pre-kurapwa. -Yakakonzerwa nekuwandisa kwemvura senitrogen kukuya, hunyoro hunowanikwa, kana huwandu hwakanyanya hwemuenzaniso.

    Mubvunzo: Maitiro ekuitisa prereatment yeiyo gDNA kuburitsa?

    Iwo ekufanofanirwa anofanira kusiyana pamasampuli akasiyana. Pamasampuli emiti, ita shuwa yekukuya zvakakwana muinitrogen yemvura. Kune sampuli yemhuka, homogenate kana kunyatso kukuya muinitrogen yemvura. Kune masampuli ane maseru maseru ayo akaoma kutyora, akadai seG + mabhakitiriya uye mbiriso, zvinokurudzirwa kushandisa lysozyme, lyticase kana mechina nzira kuputsa masero masvingo.

    Mubvunzo: Ndeupi musiyano uripo pakati pemiti mitatu yekudyara gDNA kits 4992201/4992202, 4992724/4992725, 4992709/4992710?

    4992201/4992202 Dyara Genomic DNA Kit inotora nzira-yakavakirwa nzira inoda chloroform yekubvisa. Inonyanya kukodzera kune akasiyana masampuli emiti, pamwe neyakaomeswa poda yakaoma. Hi-DNAsecure Plant Kit inoiswawo mumutsetse, asi pasina chikonzero che phenol / chloroform kuburitsa, ichiita kuti ive yakachengeteka uye isiri chepfu. Iyo inokodzera zvirimwa zvine yakakwirira polysaccharides uye polyphenol zvemukati. 4992709/4992710 DNAquick Plant System inotora nzira yemvura-yakavakirwa. Phenol / chloroform kuburitsa hazvidiwe futi. Maitiro ekuchenesa ari nyore uye anokurumidza pasina muganho wemuenzaniso wekutanga, saka vashandisi vanogona kugadzirisa huwandu hwacho nenzira inoenderana nezvinodiwa pakuedza. Kukura kukuru kwezvimedu zveGDNA zvinogona kuwanikwa nezvibereko zvakakura.

    Ndezvipi zvibereko zvinofungidzirwa zveGDNA kubva 1 ml ropa sampuro neTIANamp Ropa reDNA Kit?

    Iyo genomic DNA yakaburitswa kubva kumavhoriyamu akasiyana eropa rese revanhu neTIANamp Ropa reDNA Kit. Mhedzisiro yacho ndeinotevera. Mhedzisiro yacho yakanyorwa sereferenzi chete, iyo chaiyo mhedzisiro mhedzisiro inoenderana nemamiriro esampuli.

    faq

    Mubvunzo: 4992207/4992208 uye 4992722/4992723 ingashandiswe kubvisa ropa rakagwamba DNA?

    Ropa rinovhara ropa reDNA rinogona kuitwa pachishandiswa reagents yakapihwa mumakiti maviri aya nekungochinja protocol kune yakatarwa murau reropa rekuvhara ropa reDNA. Iyo yakapfava kopi yeropa clot DNA yekubvisa protocol inogona kupihwa pakukumbira.

    Mubvunzo: Kana uchizora TIANamp Genomic DNA Kit, maitiro ekutyora matishu matsva museru kuturika?

    Misa svumbunuro nyowani ne 1 ml PBS, yakasarudzika saline kana TE buffer. Zvakanyatso homogenize muyenzaniso ne homogenizer uye unganidza iyo inodzika pasi pezere pombi ne centrifuging. Dzinga iyo inoshamisa, uye dzorerazve iyo precipitate ne200 μl buffer GA. Iyo inotevera yekunatswa kweDNA inogona kuitwa maererano nemirairo.

    Mubvunzo: Ungasarudza sei chigadzirwa cheiyo DNA kubviswa kubva muplasma, serum uye nemuviri maseru sampu?

    Zvekucheneswa kweGDNA muplasma, serum uye muviri fluid sampuro, TIANamp Micro DNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kuSerum / plasma sampuro, TIANamp Virus DNA / RNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kune serum uye plasma sampuro, TIANamp Bacteria DNA Kit inokurudzirwa (lysozyme inofanirwa kuverengerwa kune yakanaka bhakitiriya). Zvematehwe sampu, Hi-Swab DNA Kit uye TIANamp Bacteria DNA Kit zvinokurudzirwa.

    Mubvunzo: Ungasarudza sei makiti eiyo gDNA kubviswa kubva kune fungi sampuli?

    DNAsecure Plant Kit kana DNAquick Plant System inokurudzirwa kubviswa kwefungal genome. Nezve mbiriso genome kuburitsa, TIANamp Yeast DNA Kit inokurudzirwa (lyticase inofanira kuzvigadzirira).

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