■ Nyore kushandisa: Iyo software oparesheni inogona kuve nyore kugona mukati me1 h. Iyo yakavakirwa-mukati PCR / qPCR chirongwa chekugadzirira chinogona kushandurwa uye kutapurirana nekukurumidza.
■ Zvinodyiwa zvinowirirana: Inopindirana pipette tip neBackkman Biomek 3000 system.
■ Nyore kugadzirisa: Iyo otomatiki pombi yemagetsi (APM) inogona kutsiviwa zviri nyore uye kutumirwa kumashure kuti ikanganise.
■ Kururamisa kwepamusoro uye nemazvo.
Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)
Mufananidzo 1: qPCR yakajairwa curve mhedzisiro inoratidza kudzokorora kwakanaka cDNA sampuli ye7 μl NIH 3T3 maseru akasanganiswa ka4 ne21 μl emvura pachiyero che1: 4. Roche LightCycler 480 chaiyo-nguva fluorescence yakawandisa PCR chiridzwa uye SuperReal PreMix Plus (SYBR Green) yakashandiswa pakuonekwa. |
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Mufananidzo 2: Yakanyatso nyatso kuenzaniswa neyemanyore kupomba (Kuruboshwe: Chinyorwa; Kurudyi: TEasy Automated Pipetting) 4 inodzokorora yeGAPDH yemunhu kukwidziridzwa (pamusoro curve). Wedzera 2 μl cDNA kusvika 18 μl MasterMix kuumba 20 μl reaction system. Roche LightCycler 480 chaiyo-nguva fluorescence yakawandisa PCR chiridzwa uye SuperReal PreMix Plus (SYBR Green) yakashandiswa pakuonekwa. |
Kubvira payakagadzwa, fekitori yedu yanga ichigadzira zvigadzirwa zvekutanga zvepasirese nekutevedzera mutemo
yemhando yekutanga. Zvigadzirwa zvedu zvakawana mukurumbira wakanaka muindasitiri uye yakakosha kuvimba pakati pevatengi vatsva nevakuru ..