RNAprep Yakachena Micro Kit

Yekuchenesa kwemhando yepamusoro-soro yakazara RNA kubva kune diki huwandu hweturu kana maseru.

Iyo RNAprep Yakachena Micro Kit inoshandisa yakanyatso shanda, nucleic acid-yakatarwa centrifugal adsorption ikholamu uye yakasarudzika buffer system yekubvisa nekukurumidza RNA yakazara kubva kwakasiyana siyana emhando dzakasiyana dze microsamples. Iyi kit inosanganisira Carrier RNA, iyo inogona kutora nyore nyore kutsvaga nucleic acids kubva muchirongwa. Kuburitswa kweRNA nekiti iyi kuri nyore, nekukurumidza uye kubereka nekukohwa kwakawanda. Kuita kwacho kunogona kupedzwa mukati memaminitsi makumi matatu kusvika makumi mana. Iyo kit inogona kusarudza kusarudza zvese RNA iyo iri <200 nt (5.8S rRNA, 5S rRNA uye tRNAs, nezvimwewo), ichipfumisa, kupatsanura nekuchenesa ese RNA ari> 200 nt. Iyo yakabviswa yakazara RNA yakanyanya kuchena uye isina DNA uye mapuroteni kusvibiswa.

Cat. Aihwa	Kurongedza Saizi
4992859	50 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvimiro

■ Inokwanisa yekuchenesa yemhando yepamusoro RNA kubva kune yakasarudzika masampuli, senge micro-yakadzurwa matishu, tambo ine tambo nemasero.
Uni Yakasarudzika DNase I inoderedza genomic DNA kusvibiswa.
■ Iyo yakachena-kuchena uye yakagadzirira-kushandisa RNA inokodzera kune inoyerera yekudzika mafomu.
■ Hapana phenol / chloroform yekudzinga, hapana LiCl uye ethanol kunaya, hapana CsCl gradient centrifugation inodiwa, izvo zvinoita kuti maitiro acho ave akachengeteka uye akavimbika.

Zvishandiso

■ RT-PCR.
■ Northern Blot, Dot Blot.
■ Real-Nguva PCR.
■ Chip kuongorora.
■ PolyA Kuongorora, in vitro dudziro, RNase yekudzivirira ongororo uye mamorekuru kuumbana.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: RNAsimple Yakazara RNA Kit

Zvadaro: TRNzol Yese Reagent

Yakazara RNA ye1 × 10⁶, 1 × 10⁵, 1 × 10⁴, 1 × 10³, 1 × 10², 10 Hela maseru akaburitswa achishandisa RNAprep Pure Micro Kit. RT-qPCR yakaitwa uchishandisa Quant qRT-PCR (SYBR Green) Kit yeTIANGEN.

Mubvunzo: Column blockage

A-1 Cell lysis kana homogenization haina kukwana

- Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.

A-2 Mhando yemuenzaniso yakanyanya kukura

--Deredza huwandu hwemuenzaniso wakashandiswa kana kuwedzera huwandu hwerisiti bhaisikopo.

Mubvunzo: Yakaderera RNA goho

A-1 Isina kukwana sero lysis kana homogenization

- Deredza kushandiswa kwesampula, wedzera huwandu hwerudzi rwe lysis, wedzera homogenization uye lysis nguva.

A-2 Mhando yemuenzaniso yakanyanya kukura

- Ndokumbira utarise kunonyanyisa kugadzira chinzvimbo.

A-3 RNA haina kukwidziridzwa zvizere kubva pambiru

- Mushure mekuwedzera RNase-Yemahara mvura, siya iyo kwemaminetsi mashoma pamberi pe centrifuging.

A-4 Ethanol iri mudenga

- Mushure mekuchenesa, centrifuge zvakare uye bvisa iyo yekugezera bhafa zvakanyanya sezvinobvira.

A-5 Cell tsika yepakati haina kubviswa zvachose

- Paunenge uchiunganidza maseru, ndapota ita chokwadi chekubvisa iyo tsika yepakati zvakanyanya sezvinobvira.

A-6 Masero akachengetwa muRNAstore haana kunyatsoita centrifuged

- RNAstore density yakakura kupfuura avhareji sero tsika svikiro; saka simba re centrifugal rinofanira kuwedzerwa. Inokurudzirwa kucentrifuge pa3000x g.

A-7 Yakadzika RNA zvemukati uye kuwanda mune muyenzaniso

- Shandisa yakanaka sampuro kuona kana yakaderera-goho inokonzerwa nesampuli.

Mubvunzo: RNA kudzikisira

A-1 Chinyorwa hachisi chitsva

- Nyowani nyowani inofanirwa kuchengetwa muiriidhi senitrogen nekukasira kana nekukurumidza kuiswa muRNAstore reagent kuve nechokwadi chekudzora mhedzisiro.

A-2 Mhando yemuenzaniso yakanyanya kukura

- Deredza sampuro yemari.

A-3 RNase kusvibiswan

- Kunyangwe iyo buffer yakapihwa mune kit haina RNase, zviri nyore kusvibisa RNase panguva yekubvisa uye inofanira kubatwa nehanya.

A-4 Electrophoresis kusvibiswa

- Tsiva iyo electrophoresis buffer uye ita shuwa kuti zvinoshandiswa uye Kurodha Buffer hazvina kusvibiswa neRNase.

A-5 Kunyanya kurodha electrophoresis

--Deredza huwandu hwesampuli kurodha, kurodha kwetsime rega rega hakufanirwe kupfuura 2 μg.

Mubvunzo: DNA kusvibiswa

A-1 Mhando yemuenzaniso yakanyanya kukura

- Deredza sampuro yemari.

A-2 Mimwe mienzaniso ine yakakwira DNA yezviri mukati uye inogona kurapwa neDNase.

- Ita RNase-Yemahara DNase kurapwa kune yakawanikwa RNA mhinduro, uye iyo RNA inogona kushandiswa zvakananga kuzviedza zvinotevera mushure mekurapwa, kana inogona kuvhenekerwa neRNA yekuchenesa makiti.

Mubvunzo: Ungabvisa sei RNase kubva pane zvekuyedza zvinoshandiswa nemagirazi?

Kune magirazi egirazi, akabikwa pa150 ° C kwe4 h. Pamidziyo yepurasitiki, yakanyudzwa mu0.5 M NaOH kwemaminetsi gumi, ndokunyatsogezwa nemvura isina RNase uye wozadza kuti ubvise zvachose RNase. Iwo ma reagents kana mhinduro dzinoshandiswa mukuyedza, kunyanya mvura, dzinofanirwa kunge dzisina RNase. Shandisa RNase-yemahara mvura kune ese reagent gadziriro (wedzera mvura kune yakachena girazi bhodhoro, wedzera DEPC kune yekupedzisira fungidziro ye0.1% (V / V), zunza husiku uye autoclave).

Nyora yako meseji pano uye uitumire kwatiri