RelaxGene Blood DNA System (0.1-20ml) Featured Image

RelaxGene Ropa DNA Sisitimu (0.1-20ml)

Kuburitswa kwe genomic DNA kubva pa 0.1-20 ml nyowani uye yakachengetedzwa ropa remakemikari akasiyana-siyana.

Iyo RelaxGene Ropa DNA Sisitimu inotora yakasarudzika buffer system kuburitsa genomic DNA kubva pa0-20-20 ml yeropa nyowani ine akasiyana anticoagulants uye sampuli yakachengetedzwa yeropa. Iyo buffer system inogona kubvisa mapuroteni, pigments, lipids uye zvimwe zvinodzivirira kusvibisa kusvika padanho rakakura, uye kuwana hukuru hwakakura hweDNA zvidimbu zvine goho rakakwirira, kuchena kwakanyanya uye kugadzikana kwakanaka. Iyo kititi haidi kushandisa organic manyoro anosanganisira phenol / chloroform. Iyo DNA yakanatswa inogona kushandiswa mukuyedza kwakasiyana siyana kwese sekudzivirira kugaya, PCR, kuvaka raibhurari, Maodzanyemba blot (yakakwira nzira yekuteedzera chip kusanganiswa), nezvimwe.

Cat. Aihwa	Kurongedza Saizi
4992722	50 ml
4992723	200 ml

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvimiro

■ Flexible sampuli yekutanga huwandu: 2-400 μg yakachena yakachena DNA inogona kutorwa kubva pa0.0-20 ml yemasampula eropa akasiyana.
■ Yakachengeteka uye yakavimbika: Hapana kusvibiswa kwezvisikwa zvinogadziriswa zvakadai se phenol / chloroform.

Zvishandiso

Inokodzera akasiyana siyana maitiro, akadai sekurambidzwa kugaya, PCR, kuvaka raibhurari, Maodzanyemba blot (chip kusanganiswa kweakakwira-kubudikidza kuteedzana), nezvimwe.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: TIANamp Ropa DNA Kit

Zvadaro: Hi-DNAsecure Dyara Kit

	Kucheneswa kweiyo genomic DNA kubva pa300 μl, 5 ml uye 10 ml yevanhu anticoagulation ropa neEDTA vachishandisa RelaxGene Ropa DNA System. 3 μl ye200 μl, 500 μl uye 1 ml eluates zvakateedzana zvakatakurwa pamugwagwa. M: TIANGEN Maki D15000. Mhedzisiro yekuongorora: 0.1-20 ml yeropa inogona kubviswa neRelaxGene Ropa DNA Sisitimu ine yakanaka mitsara renji.
	Kufananidza kweiyo genomic DNA yakatorwa kubva ku300 μl anticoagulation neEDTA uchishandisa RelaxGene Ropa DNA System uye zvimwe zvigadzirwa kubva kune vamwe vanopa. M: TIANGEN Maki D15000. 3 μl ye200 μl eluates akatakurwa pamugwagwa. Mhedzisiro yekuyedza: Enzanisa nevamwe vatengesi, RelaxGene Ropa DNA Sisitimu inogona kuwana gohwo repamusoro, kuchena uye kusangana kweropa gDNA.
	Genomic DNA yakabviswa mumasampuli eropa ichishandisa RelaxGene Ropa DNA System. Samples: 300 μl yevanhu anticoagulation ropa neEDTA, EDTA / NaF, heparin uye sodium citrate; buffy jasi kubva ku2 ml ropa; 10 μl yehuku yehuku uye 200 μl gonzo reropa. 3 μl ye200 μl eluates akatakurwa pamugwagwa. M: TIANGEN Maki D15000. Mhedzisiro mhedzisiro: RelaxGene Ropa DNA Sisitimu inogona kushandiswa zvakanyanya mukukwenenzverwa kwemhando yepamusoro kweDNA yemunhu anticoagulants nemishonga yakasiyana siyana pamwe neropa kubva kune akasiyana marudzi.
	Mamwe Referensi Dhata

Mubvunzo: Diki kana isina DNA mune iyo eluent.

A-1 Yakadzika kuwanda kwemasero kana hutachiona mune yekutanga sampuro -Pfumisa kuwanda kwemasero kana mavhairasi.

A-2 Kukwana kukosheswa kwemasampuli -Iyo mienzaniso haina kusanganiswa zvakaringana neye lysis buffer. Izvo zvinokurudzirwa kunyatso sanganisa ne pulse-vortexing kwe 1-2 nguva. —Iyo isina kukwana cell lysis inokonzerwa neicho chiitiko kuderera kweproteinase K. - Isina kukwana cell lysis kana protein degradation nekuda kwekushomeka kwekushambidza kwenguva yekugeza. Zvinokurudzirwa kutema zvidimbu zvidimbu zvidiki uye nekuwedzera nguva yekugeza kubvisa ese masara mu lysate.

A-3 Yakakwana DNA adsorption. -Hapana ethanol kana yakaderera-muzana panzvimbo pe100% ethanol yakawedzerwa isati yadzoserwa kune lysate kune spin column.

A-4 Iyo pH kukosha kwe elution buffer yakanyanya kuderera. - Chinja pH kuti ive pakati pe8.0-8.3.

Mubvunzo: DNA haiite mushe murwizi enzymatic reaction experiments.

Residual ethanol mune iyo eluent.

—Pane zvakasara zvekuwacha buffer PW mune yakasviba. Iyo ethanol inogona kubviswa ne centrifuging iyo spin ikholamu ye 3-5 min, uye nekuisa pakamuri tembiricha kana 50 ℃ incubator ye 1-2 min.

Mubvunzo: DNA kudzikisira

A-1 Muenzaniso hausi mutsva. -Tora muenzaniso wakanaka weDNA senzira yekuona kuti DNA iri musampu yanyadzisa here.

A-2 Isina kunaka pre-kurapwa. -Yakakonzerwa nekuwandisa kwemvura senitrogen kukuya, hunyoro hunowanikwa, kana huwandu hwakanyanya hwemuenzaniso.

Mubvunzo: Maitiro ekuitisa prereatment yeiyo gDNA kuburitsa?

Iwo ekufanofanirwa anofanira kusiyana pamasampuli akasiyana. Pamasampuli emiti, ita shuwa yekukuya zvakakwana muinitrogen yemvura. Kune sampuli yemhuka, homogenate kana kunyatso kukuya muinitrogen yemvura. Kune masampuli ane maseru maseru ayo akaoma kutyora, akadai seG + mabhakitiriya uye mbiriso, zvinokurudzirwa kushandisa lysozyme, lyticase kana mechina nzira kuputsa masero masvingo.

Mubvunzo: Ndeupi musiyano uripo pakati pemiti mitatu yekudyara gDNA kits 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Dyara Genomic DNA Kit inotora nzira-yakavakirwa nzira inoda chloroform yekubvisa. Inonyanya kukodzera kune akasiyana masampuli emiti, pamwe neyakaomeswa poda yakaoma. Hi-DNAsecure Plant Kit inoiswawo mumutsetse, asi pasina chikonzero che phenol / chloroform kuburitsa, ichiita kuti ive yakachengeteka uye isiri chepfu. Iyo inokodzera zvirimwa zvine yakakwirira polysaccharides uye polyphenol zvemukati. 4992709/4992710 DNAquick Plant System inotora nzira yemvura-yakavakirwa. Phenol / chloroform kuburitsa hazvidiwe futi. Maitiro ekuchenesa ari nyore uye anokurumidza pasina muganho wemuenzaniso wekutanga, saka vashandisi vanogona kugadzirisa huwandu hwacho nenzira inoenderana nezvinodiwa pakuedza. Kukura kukuru kwezvimedu zveGDNA zvinogona kuwanikwa nezvibereko zvakakura.

Ndezvipi zvibereko zvinofungidzirwa zveGDNA kubva 1 ml ropa sampuro neTIANamp Ropa reDNA Kit?

Iyo genomic DNA yakaburitswa kubva kumavhoriyamu akasiyana eropa rese revanhu neTIANamp Ropa reDNA Kit. Mhedzisiro yacho ndeinotevera. Mhedzisiro yacho yakanyorwa sereferenzi chete, iyo chaiyo mhedzisiro mhedzisiro inoenderana nemamiriro esampuli.

Mubvunzo: 4992207/4992208 uye 4992722/4992723 ingashandiswe kubvisa ropa rakagwamba DNA?

Ropa rinovhara ropa reDNA rinogona kuitwa pachishandiswa reagents yakapihwa mumakiti maviri aya nekungochinja protocol kune yakatarwa murau reropa rekuvhara ropa reDNA. Iyo yakapfava kopi yeropa clot DNA yekubvisa protocol inogona kupihwa pakukumbira.

Mubvunzo: Kana uchizora TIANamp Genomic DNA Kit, maitiro ekutyora matishu matsva museru kuturika?

Misa svumbunuro nyowani ne 1 ml PBS, yakasarudzika saline kana TE buffer. Zvakanyatso homogenize muyenzaniso ne homogenizer uye unganidza iyo inodzika pasi pezere pombi ne centrifuging. Dzinga iyo inoshamisa, uye dzorerazve iyo precipitate ne200 μl buffer GA. Iyo inotevera yekunatswa kweDNA inogona kuitwa maererano nemirairo.

Mubvunzo: Ungasarudza sei chigadzirwa cheiyo DNA kubviswa kubva muplasma, serum uye nemuviri maseru sampu?

Zvekucheneswa kweGDNA muplasma, serum uye muviri fluid sampuro, TIANamp Micro DNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kuSerum / plasma sampuro, TIANamp Virus DNA / RNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kune serum uye plasma sampuro, TIANamp Bacteria DNA Kit inokurudzirwa (lysozyme inofanirwa kuverengerwa kune yakanaka bhakitiriya). Zvematehwe sampu, Hi-Swab DNA Kit uye TIANamp Bacteria DNA Kit zvinokurudzirwa.

Mubvunzo: Ungasarudza sei makiti eiyo gDNA kubviswa kubva kune fungi sampuli?

DNAsecure Plant Kit kana DNAquick Plant System inokurudzirwa kubviswa kwefungal genome. Nezve mbiriso genome kuburitsa, TIANamp Yeast DNA Kit inokurudzirwa (lyticase inofanira kuzvigadzirira).

Cell Yemahara Dna Kubvisa Kit