2019-nCov Zvoga Kubvisa uye Kuona Solution naTIANGEN

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Muna Zvita 2019, akateedzana mabayo emabayo emabayo echikonzero chisingazivikanwe akatanga kubva kuWuhan, Hubei Province, uye munguva pfupi yapfuura yakapararira kumatunhu mazhinji nemaguta kuChina, nedzimwe nyika zhinji muna Ndira 2020. Kubva na22: 00 pm muna Ndira 27, 28 akasimbisa nyaya uye 5794 vanofungidzirwa 2019-nCov mhosva dzakataurwa kuChina. Iyohutachiona hunotapukira hutachiona hwakafungidzirwa saRhinolophus, uye huwandu hwerufu huripo ndi2,9%.

Musi waNdira 12, 2020, iyo World Health Organisation (WHO) yakazivisa kuti hosha yemabayo yakonzerwa nerudzi rutsva rwecoronavirus (2019-nCov). Coronavirus rudzi rwehutachiona hunopararira pakati pemhuka. Utachiona nucleic acid mhando ndeye-yakasarudzika RNA. Panguva imwecheteyo, WHO yakaburitsawo netiicic acid yekuteedzana ruzivo rweNCov yakagovaniswa nevadzidzi vechiChinese, iyo yakapa mukana wekutora mamorekuru ekuonekwa kwehutachiona uye yaita kuti kukurumidza kuona uye kuzivikanwa kwehutachiona kubvire.

WHO inoratidza kuti vanhu vane hosha dzakanyanya dzekufema dzakadai sekukosora, fivha, hutachiona hwehutachiona uye vanga vari kuWuhan mukati memazuva gumi nemana kana vakafumurwa kusangana nevamwe varwere vanofanira kuongororwa zvakazara. Musi wa17 Ndira 2020, WHO yakaburitsa iyo "Laboratory kuyedzwa kwe2019 novel coronavirus (2019-nCoV) mune fungidziro dzevanhu, nhungamiro yepakati, 17 Ndira 2020". Nhungamiro inonongedzera kuti iwo maenzaniso anofanirwa kutorwa kubva kune vanorwara zviratidzo anosanganisira ekufema masampuli (nasopharynx uye oropharyngeal swabs, sputum, bronchoalveolar lavage, nezvimwewo) uye serum specimens, seinotevera:

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* Kuti utakure masampuli ekuonekwa kwehutachiona, shandisa VTM (hutachiona hwehutachiona hwehutachiona) hune maantifungal uye maantibioti ekuwedzera. Kune yebhakitiriya kana fungal tsika, fambisa yakaoma kana mune diki kwazvo mvura isina kuchena. Dzivisa kudzokororwa kwechando nekunyungudika kwemaenzaniso.

Kunze kwezvakanangana nezvinhu zvekuunganidza zvinoratidzwa mutafura zvakare vimbisa zvimwe zvigadzirwa nemidziyo zviripo: eg midziyo yekufambisa uye masampuli ekuunganidza mabhegi uye kurongedza, zvinotonhora uye mapakeji anotonhorera kana chando chakaoma, michina yekuchera ropa isina kuchena (semuenzaniso tsono, majekiseni uye machubhu), zvinyorwa uye zvechigarire mamaki, PPE, zvigadzirwa zvekusvibisa nzvimbo.

Zvekutsvaga hutachiona, serum specimens inogona kushandiswa kutsvaga hutachiona nenzira yekuzvidzivirira muviri, nepo RTqPCR ichikurudzirwa kuona nCov nucleic acid kuti ikurumidze kuona uye nekujeka. Sezvo hutachiona
akateedzana anozivikanwa, zvirimo zvehutachiona nucleic acid mune iyo specimen inogona kuonekwa nekukasira chete
kusarudza ma reagents akakodzera uye enzanisa ma primers

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(Kuongorora kuongororwa kweWuhan coronavirus 2019 neiyo chaiyo-nguva RTPCR, Protocol uye kuongorora kwekutanga saJan 13, 2020) Semutungamiri mumunda wekubvisa nucleic acid uye kuona muChina, TIANGEN BIOTECH (BEIJING) CO., LTD. yakapa hutachiona-ruoko-muromo uye hutachiona A (H1N1) hutachiona hwekuona hutachiona hwevanhu vanopfuura mamirioni gumi. Mu2019, TIANGEN's hutachiona nucleic acid kuburitsa uye kuona ma reagents akaiswa kumamirioni makumi matatu eAfrica echinyakare swine fever sampuli, zvichipa zvakanakisa kuongororwa uye kudzivirira kweAfrican classical swine fever muChina. TIANGEN haingopa chete kukurumidza uye kwakaringana hutachiona hwekubvisa uye kuona reagents, asi zvakare inopa zvirinyore uye zvinobatika zvinyama zvekukuya zviridzwa uye otomatiki nucleic acid madhizaini, ayo anoumba seti yakazara yegadziriro yehutachiona kubviswa uye kuona.

Zvoga Nucleic Acid Extraction Solution

TIANGEN Automated Nucleic Acid Extractors ndiwo otomatiki mapuratifomu eiyo nucleic acid kuburitsa vachishandisa magnetic bead nzira. Kuiswa kwemapuratifomu aya hakungoderedze zvakanyanya kuwanda kwebasa rekutarisa nekuisa dhipatimendi, asi zvakare kunoderedza zvikanganiso zvekushandisa kwekuburitsa bhuku, uye kunovimbisa kusagadzikana uye kugadzikana kwehunhu hwekuburitsa nucleic acid.

TIANGEN Automated Nucleic Acid Extractor ine nzira dzakasiyana siyana (kusanganisira 16, 24, 32, 48, 96 chaneli), uye ma reagents anoenderana anogona kushandiswa kune iyo nucleic acid kuburitsa kubva pamhando dzakasiyana dzemhando. TIANGEN inopawo yakasarudzika reagent kuvandudza uye chiridzwa kusangana masevhisi zvinoenderana akasiyana ekuyedza anodikanwa.

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TGrinder H24 Tissue Homogenizer

● Inoshandiswa zvakanyanya kukuya uye homogenizing maturusi akasiyana-siyana netsvina yetsvina
● Nokukuya homogenization simba 2-5 zvakapetwa izvo zvechinyakare zviridzwa
● Uniform kukuya uye homogenization, kudzivirira muchinjikwa kusvibiswa
● Chishandiso chekudzivirira otomatiki kuchengetedza kuchengetedzeka kwevashandi vema laboratori

TGuide S32 Yakagadzirwa Nucleic Acid Inobvisa

● Sample throughput: 1-32 samples
● Kugadzira vhoriyamu: 20-1000 μl
● Mhando yemhando: Ropa, masero, nyama, mutsvina, hutachiona uye mamwe masampuli
● Nguva yekugadzirisa: Kusvika pamaminetsi masere kuti uwane hutachiona nucleic acid
● Kudzora maitiro: Dual control mode yeWindows Pad uye bhatani rekrini
● Kukura kwepuratifomu: Vhura chikuva, chemahara kuenzanisa reagents

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TGuide S32 Magnetic Viral DNA / RNA Kit (DP604)
● Wide application: Yakakwira-mhando hutachiona DNA / RNA inogona kucheneswa kubva kune serum, plasma, swab sampuro, mishonga yekurapa mhinduro uye dzakasiyana siyana hutachiona hwekuchengetedza mhinduro.
● Zviri nyore uye zvinobudirira: Ichi chigadzirwa chakanyatsoenderana neTGuide S32 Automated Nucleic Acid Extractor, iyo inogona kubvisa hutachiona DNA / RNA ine goho rakakura, yakachena kuchena, yakatsiga uye yakavimbika mhando.
● Maodzanyemba ekushandisa: Iyo yakanatswa nucleic acid inokodzera yekudzika kuyedzwa kwehutachiona PCR uye chaiyo-nguva PCR.

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Manual Nucleic Acid Kubvisa Solution

Semutungamiri mumunda weiyo nucleic acid kuburitsa uye kuona, TIANGEN ine yakanyanya yakazara nhevedzano yezvigadzirwa zveNicic acid kudhizaina kuChina, ayo akakodzera kuti coronavirus ionekwe kubva kumarudzi ese esampuli: ropa, serum / plasma, tishu, swab, hutachiona , nezvimwe.

TIANamp Virus DNA / RNA Kit (DP315)

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● Kubudirira kwepamusoro: Yakakwira mhando hutachiona DNA neRNA inogona kuwanikwa nekukurumidza kunatsa nekukwirazve kudzokorora.
● Kuchena kwakanyanya: Kubvisa zvakakwana zvinosvibisa uye zvinodzivirira zvekushandisa pazasi.
● Kuchengetedzwa kwepamusoro: Organic reagent kuburitsa kana ethanol kunaya hazvidiwe.

RNA Virus Yekutsvaga Solution

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1.Teasy Michina Pipetting Sisitimu
● Kurongeka kwepamusoro: Iyo inotonhorera block inogona kuchengetedza tembiricha ye reagent sampu pazasi pe7 ℃ kweanopfuura makumi matanhatu maminitsi. Imwe neimwe APM inoenzanisirwa zvinoenderana nematanho epasi rose, ayo ane huchokwadi hwakanyanya kupfuura hwemanyorero kupomba.
● Kushanda kuri nyore: Saizi diki. Chiedza chechiedza. Hapana chishandiso chinodiwa che block block kutsiva. Yakavakwa-muPCR / qPCR maitiro ekugadzirira. Yakareruka mashandiro ekushandisa kwePCR mhinduro yekugadzirira.
● Wide application: Inogona kushandiswa kune 96/384-tsime ndiro kupomba, PCR, qPCR, kutsvaga kwejini uye kumwe kuyedzwa kwepamusoro-soro.

 FastKing Imwe Nhanho RT-qPCR Kit (Probe) (FP314)

Iyo FastKing Imwe Nhanho RT-qPCR Kit (Probe) (FP314) inogadzirwa neTIANGEN ndeye onestep reverse transcript fluorescence chiyero chekutora kit chinoenderana nenzira yekuferefeta, iyo yakanyatsogadzirirwa kuti utsvage maronda emajini mumasampuli akasiyana. Iyo KingRTase mune kit iri nyowani mamorekuru akagadziridzwa reverse transcriptase, ine yakasimba RNA kuwirirana uye kupisa kwemafuta, nekuvandudzwa kweshure kunyorwa kwekunyatsoshanda uye nekuwedzera kugona kweakaomarara sekondari chimiro RNA matemplate. Kutanga kutsva kutsva Taq DNA polymerase kunoiswawo kupa iyo PCR reaction yepamusoro yekukwirisa kugona uye kusarongeka. Uyezve, kit yacho inorerutsa zvinhu kusvika pamwero mukuru nekusanganisa enzyme yeTaq neMLV kuita enzyme musanganiswa, uye pre-kusanganisa ion buffer, dNTPs, PCR stabilizer uye inosimudzira kuva MasterMix, kuitira kuti nhanho-yekusanganisa matanho ive
chakareruka.
● Kubudirira kwepamusoro: Iyo yakanakisa reverse transcriptase uye DNA polymerase inoona yakanyanya kuita zvine mutsindo
● Kudzoreredza kwakanaka: Iyo polymerase inogona kuverenga kuburikidza neiyo RNA template ine yakakwira GC zvemukati uye yakaoma yechipiri chimiro
● Wide mafomu: Kumusoro kushanda kune maRNA matemplate ane tsvina kubva kune akasiyana marudzi
● Kunzwisisa kwakanyanya: Matemplate akaderera se1 ng anogona kunyatso kuzivikanwa, kunyanya kune mashoma mashoma matemplate

Muenzaniso weRNA Virus Detection

Iyo nucleic acid yeH5 avian furuwenza yakatorwa neTGuide S32 Magnetic Viral DNA / RNA Kit (DP604). Iyo FastKing Imwe Nhanho RT-qPCR Kit (Probe) (FP314) yakashandiswa pakuonekwa kweRT-qPCR ichishandisa yakatarwa H5 avian furuwenza primers uye ma probes.

ABi7500Fast yakashandiswa pakuonekwa kweRT-qPCR. Mhedzisiro yeH5 avian influenza hutachiona antigen (10-2, 10-3, 10-4, 10-5, 10-6 uye 10-7 dilution) kubva ku200 μl sampu inoratidza yakakwira hutachiona nucleic acid kuburitsa goho, iyo inogona kusangana neinotevera zvido zvekudzosera zvinyorwa, PCR, RT-PCR, chaiyo-nguva PCR, nezvimwe. Iyo chaiyo-nguva PCR inounza mhedzisiro nekukwirisa kwekunzwa, kudzokorora kwakanaka uye kwakanaka gradient dilution linearity. Kusiyanasiyana kwakasiyana kwehutachiona nucleic acid sampuli kunogona kunyatsoonekwa.

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Post nguva: Kubvumbi-11-2021