Magnetic Animal Tissue Genomic DNA Kit

Kusurukirwa uye kunatswa kwemhando yepamusoro genomic DNA kubva kumhuka dzemhuka.

Iyo kitsi inotora magineti mhete ine yakasarudzika yekuparadzanisa basa uye yakasarudzika buffer system yekuparadzanisa nekuchenesa yemhando yepamusoro genomic DNA kubva kumhuka tishu sampuli. Yakasarudzika yakaiswa magineti mabheji ane hukama hwakasimba hweiyo nucleic acid pasi pemamwe mamiriro. Kana mamiriro acho achinge achinja, iwo magineti marozari achaburitsa adsorbed nucleic acid, zvichizadzisa chinangwa chekukurumidza kupatsanura uye kuchenesa kweiyo nucleic acid. Maitiro ese akachengeteka uye ari nyore. Iine zvidimbu zvakakura zvakaburitswa genomic DNA zvidimbu, kuchena kwakanyanya uye mhando yakavimbika, nzira yacho inonyanya kukodzera otomatiki kudhiza kwepamusoro-throughput nzvimbo dzekushandira.

Cat. Aihwa	Kurongedza Saizi
4992404	50 preps
4992405	200 preps
4992864	1000 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

MIBVUNZO

Chigadzirwa Mateki

Zvimiro

■ Zviri nyore uye zvinomhanya: Direct lysis isina incubation, uye ultrapure genomic DNA inogona kuwanikwa mukati meawa rimwe chete.
■ Kubudirira kwepamusoro: Inogona kuverengerwa nemidziyo yekushandisa yeipayipi nzira uye yemagineti tsvimbo nzira yekuyedza kwepamusoro kuburitsa kwekuyedza.
■ Yakachengeteka uye isina chepfu: Hapana maekisesaidhi ehupenyu akaita se phenol / chloroform anodikanwa.
■ Kuchena kwepamusoro: Iyo DNA yakawanikwa ine kuchena kwakanyanya uye inogona kushandiswa yakanangana mukubata chip, kurongedza kwepamusoro-soro uye kumwe kuyedza.

Zvishandiso

Iyo yakanatswa DNA inogona kuiswa kune akasiyana echinyakare kuyedza senge enzyme kugaya, PCR, raibhurari yekuvaka, Maodzanyemba masanganiswa, chip kusanganiswa, kukwirisa-kwepasuru kurongeka, nezvimwe.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: Serum / Plasma Inotenderera DNA Kit

Zvadaro: Magnetic Ropa Spots DNA Kit

Shandisa Magnetic Animal Tissue Genomic DNA Kit kuchenesa DNA kubva kumatunhu akasiyana emakonzo, hove, shrimp, shellfish, maseru akagadzirwa, nyama yegumbo redhadha uye mimwe mienzaniso maererano nemirairo. M: TIANGEN Maki D15000.
Elution vhoriyamu iri 150 µl. 1-2 elul eluent yakaiswa paagarose gel electrophoresis.
Mhedzisiro: TIAGNEN Magnetic Animal Tissue Genomic DNA Kit ine yakawanda yekuenzanisira kushandiswa, kunyanya kune akaomarara masampula senge gonzo muswe, hove, shrimp uye shellfish.

Shandisa TIAGNEN Magnetic Animal Tissue Genomic DNA Kit uye akakodzera makiti eMutengesi M, O, A kuchenesa DNA kubva ku25 mg rat rat moyo tishu maererano nemirairo. M: TIANGEN Maki D15000.
Elution vhoriyamu: 150 .l. 1 elul eluent yakaiswa pane agarose gel electrophoresis.
Mhedzisiro: TIAGNEN Magnetic Animal Tissue Genomic DNA Kit ine yakakwira yekuchera goho.

Mubvunzo: Diki kana isina DNA mune iyo eluent.

A-1 Yakadzika kuwanda kwemasero kana hutachiona mune yekutanga sampuro -Pfumisa kuwanda kwemasero kana mavhairasi.

A-2 Kukwana kukosheswa kwemasampuli -Iyo mienzaniso haina kusanganiswa zvakaringana neye lysis buffer. Izvo zvinokurudzirwa kunyatso sanganisa ne pulse-vortexing kwe 1-2 nguva. —Iyo isina kukwana cell lysis inokonzerwa neicho chiitiko kuderera kweproteinase K. - Isina kukwana cell lysis kana protein degradation nekuda kwekushomeka kwekushambidza kwenguva yekugeza. Zvinokurudzirwa kutema zvidimbu zvidimbu zvidiki uye nekuwedzera nguva yekugeza kubvisa ese masara mu lysate.

A-3 Yakakwana DNA adsorption. -Hapana ethanol kana yakaderera-muzana panzvimbo pe100% ethanol yakawedzerwa isati yadzoserwa kune lysate kune spin column.

A-4 Iyo pH kukosha kwe elution buffer yakanyanya kuderera. - Chinja pH kuti ive pakati pe8.0-8.3.

Mubvunzo: DNA haiite mushe murwizi enzymatic reaction experiments.

Residual ethanol mune iyo eluent.

—Pane zvakasara zvekuwacha buffer PW mune yakasviba. Iyo ethanol inogona kubviswa ne centrifuging iyo spin ikholamu ye 3-5 min, uye nekuisa pakamuri tembiricha kana 50 ℃ incubator ye 1-2 min.

Mubvunzo: DNA kudzikisira

A-1 Muenzaniso hausi mutsva. -Tora muenzaniso wakanaka weDNA senzira yekuona kuti DNA iri musampu yanyadzisa here.

A-2 Isina kunaka pre-kurapwa. -Yakakonzerwa nekuwandisa kwemvura senitrogen kukuya, hunyoro hunowanikwa, kana huwandu hwakanyanya hwemuenzaniso.

Mubvunzo: Maitiro ekuitisa prereatment yeiyo gDNA kuburitsa?

Iwo ekufanofanirwa anofanira kusiyana pamasampuli akasiyana. Pamasampuli emiti, ita shuwa yekukuya zvakakwana muinitrogen yemvura. Kune sampuli yemhuka, homogenate kana kunyatso kukuya muinitrogen yemvura. Kune masampuli ane maseru maseru ayo akaoma kutyora, akadai seG + mabhakitiriya uye mbiriso, zvinokurudzirwa kushandisa lysozyme, lyticase kana mechina nzira kuputsa masero masvingo.

Mubvunzo: Ndeupi musiyano uripo pakati pemiti mitatu yekudyara gDNA kits 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Dyara Genomic DNA Kit inotora nzira-yakavakirwa nzira inoda chloroform yekubvisa. Inonyanya kukodzera kune akasiyana masampuli emiti, pamwe neyakaomeswa poda yakaoma. Hi-DNAsecure Plant Kit inoiswawo mumutsetse, asi pasina chikonzero che phenol / chloroform kuburitsa, ichiita kuti ive yakachengeteka uye isiri chepfu. Iyo inokodzera zvirimwa zvine yakakwirira polysaccharides uye polyphenol zvemukati. 4992709/4992710 DNAquick Plant System inotora nzira yemvura-yakavakirwa. Phenol / chloroform kuburitsa hazvidiwe futi. Maitiro ekuchenesa ari nyore uye anokurumidza pasina muganho wemuenzaniso wekutanga, saka vashandisi vanogona kugadzirisa huwandu hwacho nenzira inoenderana nezvinodiwa pakuedza. Kukura kukuru kwezvimedu zveGDNA zvinogona kuwanikwa nezvibereko zvakakura.

Ndezvipi zvibereko zvinofungidzirwa zveGDNA kubva 1 ml ropa sampuro neTIANamp Ropa reDNA Kit?

Iyo genomic DNA yakaburitswa kubva kumavhoriyamu akasiyana eropa rese revanhu neTIANamp Ropa reDNA Kit. Mhedzisiro yacho ndeinotevera. Mhedzisiro yacho yakanyorwa sereferenzi chete, iyo chaiyo mhedzisiro mhedzisiro inoenderana nemamiriro esampuli.

Mubvunzo: 4992207/4992208 uye 4992722/4992723 ingashandiswe kubvisa ropa rakagwamba DNA?

Ropa rinovhara ropa reDNA rinogona kuitwa pachishandiswa reagents yakapihwa mumakiti maviri aya nekungochinja protocol kune yakatarwa murau reropa rekuvhara ropa reDNA. Iyo yakapfava kopi yeropa clot DNA yekubvisa protocol inogona kupihwa pakukumbira.

Mubvunzo: Kana uchizora TIANamp Genomic DNA Kit, maitiro ekutyora matishu matsva museru kuturika?

Misa svumbunuro nyowani ne 1 ml PBS, yakasarudzika saline kana TE buffer. Zvakanyatso homogenize muyenzaniso ne homogenizer uye unganidza iyo inodzika pasi pezere pombi ne centrifuging. Dzinga iyo inoshamisa, uye dzorerazve iyo precipitate ne200 μl buffer GA. Iyo inotevera yekunatswa kweDNA inogona kuitwa maererano nemirairo.

Mubvunzo: Ungasarudza sei chigadzirwa cheiyo DNA kubviswa kubva muplasma, serum uye nemuviri maseru sampu?

Zvekucheneswa kweGDNA muplasma, serum uye muviri fluid sampuro, TIANamp Micro DNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kuSerum / plasma sampuro, TIANamp Virus DNA / RNA Kit inokurudzirwa. Zvekucheneswa kwehutachiona gDNA kubva kune serum uye plasma sampuro, TIANamp Bacteria DNA Kit inokurudzirwa (lysozyme inofanirwa kuverengerwa kune yakanaka bhakitiriya). Zvematehwe sampu, Hi-Swab DNA Kit uye TIANamp Bacteria DNA Kit zvinokurudzirwa.

Mubvunzo: Ungasarudza sei makiti eiyo gDNA kubviswa kubva kune fungi sampuli?

DNAsecure Plant Kit kana DNAquick Plant System inokurudzirwa kubviswa kwefungal genome. Nezve mbiriso genome kuburitsa, TIANamp Yeast DNA Kit inokurudzirwa (lyticase inofanira kuzvigadzirira).

Cell Yemahara Dna Kubvisa Kit