EndoFree Mini Plasmid Kit II

Yekuchenesa kweiyo endotoxin-yemahara yekuisa giredhi plasmid DNA.

Iyo EndoFree Mini Plasmid Kit II inotora yakasarudzika silica membrane adsorption tekinoroji kunyatsoita uye kunyatso kusunga plasmid DNA. Iyo yakakosha endotoxin-inobvisa system inogona kunyatso bvisa marara senge endotoxin nemapuroteni. Iyo yese yekubvisa maitiro inotora chete 1 awa, iri nyore uye nekukurumidza. Chibereko cheplasmid chakaburitsa chakabatana neplasmid kopi nhamba, inomiririra mabhakitiriya mhando uye mamiriro etsika. Kusvikira ku 70 μg yeplasmid DNA inogona kubviswa kubva pa5-15 ml yehusiku hwakagadziriswa hutachiona hutsika hwepakati.

Cat. Aihwa	Kurongedza Saizi
4992422	50 preps

Tumira neindaneti kwatiri

Chigadzirwa Chigadzirwa

Muenzaniso Wekuedza

Chigadzirwa Mateki

Zvimiro

■ Kumhanyisa uye kwakakura goho: 5-70 μg plasmid DNA inogona kuburitswa mukati rinenge awa rimwe.
■ Yakachena-kuchena: Iyo yakakosha endotoxin kubvisa system yakasanganiswa ne Spin Column CP4 inogona chaizvo kushambadza nucleic acid uye kubvisa endotoxin kune yakaderera chikamu.
W Zvikamu zvakasiyana-siyana zvekushandisa: Inokodzera kuyedza kwemazuva ese sekudzivirira endonuclease kugaya, PCR, kuteedzana, ligation, shanduko, pamwe nekuyedza kwepamusoro-soro kwakadai sekurapa kwemajini, sero microinjection, kudzikamisa majini, kunyora, nezvimwe.

Kubereka kwePlasmid

Plasmid Yield

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)

Pashure: EndoFree Maxi Plasmid Kit

Zvadaro: TIANprep N96 Magnetic Plasmid Kit

Inokodzera kuburitswa kwepamusoro / pasi kopi plasmids
Mufananidzo 1. Kubviswa kweplasmid DNA kubva kwakasiyana siyana vhoriyamu yebhakitiriya tsika yepakati uchishandisa EndoFree Mini Plasmid Kit II.
Elution vhoriyamu: 200 μl; Kuisa vhoriyamu: 3 μl yakaderera-kopi plasmid pBR322, 2 μl yakakwira-kopi plasmid pBS.
Iyo electrophoresis yakaitwa pa6 V / cm kwemaminitsi makumi matatu pa1% agarose.
Kuwedzeredzwa kwesero neiyo yakanatswa plasmid

Mufananidzo 2 Iyo PEGFP plasmid yakanatswa neEndoFree Mini Plasmid Kit II yakaendeswa mumaseru e293T. Tsananguro yeGFP yakaonekwa mu48 h post-transfection

Nyora yako meseji pano uye uitumire kwatiri