EndoFree Maxi Plasmid Kit V2

Kunatswa kwe endotoxin-yemahara kutapurika giredhi plasmid DNA yakanangana nemaseru anononoka.

Iyo EndoFree Maxi Plasmid Kit V2 inotora yakasarudzika silika membrane kushambadzira matekiniki kuti ibudirire uye yakanyatso kusunga plasmid DNA. Iyo yakakosha Buffer ER uye iyo Filtration CS1 inogona zvinobudirira kubvisa tsvina senge endotoxin uye protein. Iyo yose yekubvisa maitiro inotora chete 1 awa.
Yakakurudzira mabhakitiriya tsika yepakati huwandu: Kune yakakwira-kopi plasmid, 100 ml mabhakitiriya epakati anokurudzirwa, ayo anogona kuburitsa anosvika 500-1500 μg plasmid. Kune yakaderera-kopi plasmid, 200 ml yebhakitiriya yepakati inokurudzirwa kuti uwane goho repakati pe50-300 μg.

Cat. Aihwa Kurongedza Saizi
4992438 10 preps

Chigadzirwa Chigadzirwa

Kufamba kwebasa

Muenzaniso Wekuedza

Chigadzirwa Mateki

Zvimiro

■ Kuchena kwakakwirira: Iyo yakasarudzika endotoxin kunaya tekinoroji inogamuchirwa kuti ibvise zvakananga endotoxin.
■ Nyore kushanda: Iyo adsorption column tekinoroji inoshandiswa kunyatso adsorb plasmid DNA, iyo inoita kuti kuvhiya kuve nyore.
High Kutenderera kwepamusoro-soro: Inokodzera kutapuriranwa kwemasero mazhinji anosanganisira maseru anonzwisisa endotoxin.
Range Mhando dzakasiyana dzekushandisa: Iyo plasmid yakanatswa inogona kuiswa mukutapurirana kwemasero emhuka uye emiti pamwe nekuyedza kwemoreniyamu biology.

Zvishandiso

Iyo plasmid DNA yakaburitswa ichishandisa kit iyi inogona kushandiswa pamabasa akasiyana siyana, kusanganisira kudzora enzyme kugaya, PCR, kuteedzana, ligation, shanduko uye kutapurika kwemasero akasiyana.

Zvese zvigadzirwa zvinogona kuve zvakagadziriswa ODM / OEM. Kuti uwane mamwe mashoko,ndokumbira ubaye Customized Service (ODM / OEM)


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    Workflow

    Experimental Example 图片 2 Iyo yakasarudzika endotoxin kubvisa reagent Buffer ER inogona kunyatso bvisa endotoxin masara mune reaction system, uye yakakwira-yakachena plasmid inogona kuwanikwa. Iyo plasmid endotoxin masara iri ≤ 0.1 EU / ml.
    Experimental Example Iyo plasmid yakanatswa ichishandisa EndoFree Maxi Plasmid Kit V2 uye izvo zvakafanana zvigadzirwa kubva kuMutengesi 1 uye Mutengesi 2 zvakashongedzwa nevhoriyamu yakafanana yeelution buffer. 1 μl plasmid yakatakurwa pamugwagwa wega wega kufungidzira kuwanda kweplasmid. 100 ng yeplasmid iyo kusangana kwayo kwakatemwa ne spectrophotometer yakaiswa mune imwechete gel kuti ione kana kukosha kwevasungwa kwaive kwekunyepa kwepamusoro.
    Mhedzisiro
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